Anti-Aurora A (phospho T288) 抗体 (ab18318)


  • 製品名
    Anti-Aurora A (phospho T288) antibody
    Aurora A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Aurora A (phospho T288)
  • 特異性
    This antibody has been shown to mildly react with Aurora B if excess antibody is applied (cross-reactivity with Aurora B can be observed in immunofluorescence)
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide: SRRTTPLCGT, corresponding to amino acids 284-292 of Human Aurora A

  • ポジティブ・コントロール
    • Nocadozole-arrested HeLa cell lysate immunoprecipitate with a pan-Aurora A monoclonal antibody (clone 35C1)(ab13824)
  • 特記事項
    Hela cells were synchronized to M phase using nocadazole (18 hrs treatment). Mitotic shake-off cells were used to prepare extracts. For a 10 cm2 dish (approximately 80% confluent, about 60% in M-phase), typically 2-4 lanes would be loaded for Western blot. Phosphorylated Aurora A can be difficult to detect, you may want to IP first with pan-Aurora A before blotting with the phospho-specific antibody.



Our Abpromise guarantee covers the use of ab18318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). It is strongly recommended that immunoprecipitation with a pan-specific Aurora antibody be carried out before Western blotting with the phospho-specific antibody to increase signal. Weak non specific bands can be sometimes observed above the band of interrest. A large non specific band is also present just above 32kDa.
ICC/IF 1/500 - 1/1000.


  • 機能
    Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
  • 組織特異性
    Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
  • 配列類似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
  • 細胞内局在
    Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AIK antibody
    • ARK-1 antibody
    • ARK1 antibody
    • AURA antibody
    • Aurka antibody
    • Aurora 2 antibody
    • Aurora A antibody
    • Aurora kinase A antibody
    • Aurora-related kinase 1 antibody
    • Aurora/IPL1 like kinase antibody
    • Aurora/IPL1-related kinase 1 antibody
    • AURORA2 antibody
    • Breast tumor-amplified kinase antibody
    • BTAK antibody
    • hARK1 antibody
    • IAK antibody
    • IPL1 related kinase antibody
    • MGC34538 antibody
    • OTTHUMP00000031340 antibody
    • OTTHUMP00000031341 antibody
    • OTTHUMP00000031342 antibody
    • OTTHUMP00000031343 antibody
    • OTTHUMP00000031344 antibody
    • OTTHUMP00000031345 antibody
    • OTTHUMP00000166071 antibody
    • OTTHUMP00000166072 antibody
    • PPP1R47 antibody
    • Protein phosphatase 1, regulatory subunit 47 antibody
    • Serine/threonine kinase 15 antibody
    • Serine/threonine kinase 6 antibody
    • Serine/threonine-protein kinase 15 antibody
    • Serine/threonine-protein kinase 6 antibody
    • Serine/threonine-protein kinase aurora-A antibody
    • STK15 antibody
    • STK6 antibody
    • STK6_HUMAN antibody
    • STK7 antibody
    see all

Anti-Aurora A (phospho T288) antibody 画像

  • Predicted band size : 46 kDa
    Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were immunoprecipitated with a pan-Aurora A monoclonal antibody (clone 35C1) (available as ab13824), resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal antibody against Thr288 phosphorylated Aurora A (ab18318). Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP (available as ab16284), and a chemiluminescence detection system

  • Predicted band size : 46 kDa
    The blot shown above was stripped and reprobed with pan-Aurora specific polyclonal antibody (available as ab12875) to verify equal loading of Aurora A in the extracts. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP (available as ab16284)and a chemiluminescence detection system.
  • Immunofluorescence analysis of P. mammillata embryos during mitotic cycle, staining Aurora A (phospho T288) with ab18318.

    Embryos were fixed in methanol/1%formaldehyde (-20°C) overnight, permeabilized in 0.25% Triton X-100 for 15 minutes at room temperature and then incubated in primary antibody (1/200) in PBS/1%BSA overnight at 4°C. A FITC-conjugated anti-rabbit IgG (1/50) was used as the secondary antibody.
  • Prometaphase HeLa cells were permaeabiliased in 0.2% Triton-X100 and fixed for 10 minutes at room temperature in 4% formaldehyde. The cells were washed 3 times in PBS and incubated with ab18318 (1/1000) for 1 hour at room temperature. The panel of images shows the nuceli stained with DAPI (blue) and the centromeres stained with ab18318 (green). 100x magnification.

Anti-Aurora A (phospho T288) antibody (ab18318) 使用論文

This product has been referenced in:
  • Morris EJ  et al. Stat3 regulates centrosome clustering in cancer cells via Stathmin/PLK1. Nat Commun 8:15289 (2017). FM ; Human . Read more (PubMed: 28474672) »
  • Braun A  et al. Rac1 and Aurora A regulate MCAK to polarize microtubule growth in migrating endothelial cells. J Cell Biol 206:97-112 (2014). Read more (PubMed: 25002679) »

See all 10 Publications for this product

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Thank you for contacting us. I have attached the datasheet as requested. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam has not validated the combination of species/application used in this Abreview.
Human Recombinant protein (Kinase domain)
Kinase domain
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C

Dr. Charlotte Dodson

Verified customer

投稿 Nov 30 2010

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab18318 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support but please ...

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I received this morning the reply from the laboratory that tested ab18318 who provided me with very useful information: "In looking at the blot that your customer sent, it looks like the lower band that they are seeing is a non-specific band that we...

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Once again, thank you for your enquiry. I have been in touch with the originator of this antibody. We have discussed the results that your customer has been having. The originator of this antibody has also passed the results onto one of their collab...

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Thank you for your enquiry. Once again I am sorry to hear that your customer has been having difficulties with this antibody. This antibody was in fact tested by an external collaborator. I would appreciate it if you could request some images f...

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Thank you for your enquiry. I am able to confirm that the buffer constituents are PBS containing 0.09% sodium azide and 50% glycerol only no other protein stabilizers are present. I hope that this information is useful? Best of luck with your research.

Thank you for your enquiry. The sequence of the immunizing peptide is as follows: SRRTT*LCGT I hope this information helps. Please do not hesitate to contact us again if you need anything further.

Thank you for your enquiry. Thank you for sending the pictures of your blots as well. I am convinced from your blots that the antibody is recognizing phosphorylated Aurora A and not unphosphorylated Aurora A. This is clear when you are using milk. ...

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Thank you for your enquiry. The latest LOT of this antibody is 0.45 mg/ml. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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