製品の概要

  • 製品名Anti-Aurora A antibody [35C1]
    Aurora A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [35C1] to Aurora A
  • 特異性The specificity of ab13824 was confirmed by showing that it binds in ELISA to recombinant Aurora A, it detects a 46kd band on HeLa cell extract and it binds to duplicated centrosomes and spindle poles in MCF7 cells. This antibody does not inhibit the kinase activity of Aurora A. This antibody has been successfully used to detect Aurora A in the following cell extracts: 293T, HMEC, T47D, MCF7, MDA-MB-468, SK-Br-3, S68 and HeLa.
  • アプリケーション適用あり: WB, ELISA, ICC, ICC/IF, IP, IHC-Fr, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant full length his-tagged Aurora A protein (Human).

  • ポジティブ・コントロール
    • Human HeLa and mouse M-ICc12 cell lysates for Western blotting and human 293 or mouse LLC1 cell lines for IF. In Flow Cytometry, this antibody gave a positive signal in HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa.
ELISA Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 2µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
  • 組織特異性Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
  • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
  • 細胞内局在Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AIK antibody
    • ARK-1 antibody
    • ARK1 antibody
    • AURA antibody
    • Aurka antibody
    • Aurora 2 antibody
    • Aurora A antibody
    • Aurora kinase A antibody
    • Aurora-related kinase 1 antibody
    • Aurora/IPL1 like kinase antibody
    • Aurora/IPL1-related kinase 1 antibody
    • AURORA2 antibody
    • Breast tumor-amplified kinase antibody
    • BTAK antibody
    • hARK1 antibody
    • IAK antibody
    • IPL1 related kinase antibody
    • MGC34538 antibody
    • OTTHUMP00000031340 antibody
    • OTTHUMP00000031341 antibody
    • OTTHUMP00000031342 antibody
    • OTTHUMP00000031343 antibody
    • OTTHUMP00000031344 antibody
    • OTTHUMP00000031345 antibody
    • OTTHUMP00000166071 antibody
    • OTTHUMP00000166072 antibody
    • PPP1R47 antibody
    • Protein phosphatase 1, regulatory subunit 47 antibody
    • Serine/threonine kinase 15 antibody
    • Serine/threonine kinase 6 antibody
    • Serine/threonine-protein kinase 15 antibody
    • Serine/threonine-protein kinase 6 antibody
    • Serine/threonine-protein kinase aurora-A antibody
    • STK15 antibody
    • STK6 antibody
    • STK6_HUMAN antibody
    • STK7 antibody
    see all

Anti-Aurora A antibody [35C1] 画像

  • All lanes : Anti-Aurora A antibody [35C1] (ab13824) at 5 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 125 kDa (possible non-specific binding),55 kDa (possible non-specific binding).

    Exposure time : 20 minutes

    This blot was produced using 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200v for 50 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab13824 over night at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.

  • ab13824, at 1/2000 dilution, detecting Aurora A (green) in Hela Cells in conjunction with a Goat anti-mouse secondary antibody conjugated to Cy3®. Cells were fixed with methanol and counterstained with DAPI. Please refer to abreview for further details.

    See Abreview

  • ICC/IF image of ab13824 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13824, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Overlay histogram showing HeLa cells stained with ab13824 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13824, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-Aurora A antibody [35C1] (ab13824) 使用論文

This product has been referenced in:
  • Huang CJ  et al. The SUMO Protease SENP3 Orchestrates G2-M Transition and Spindle Assembly in Mouse Oocytes. Sci Rep 5:15600 (2015). Mouse . Read more (PubMed: 26493771) »
  • Cho MK  et al. Elevated aurora kinase a protein expression in diabetic skin tissue. Arch Plast Surg 41:35-9 (2014). IHC-P ; Human . Read more (PubMed: 24511492) »

See all 23 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (Brain prefrontal cortex)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 15 µg
Specification Brain prefrontal cortex
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Oct 14 2016

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Huh7 (liver))
Specification Huh7 (liver)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Caroline Gleason

Verified customer

投稿 Apr 30 2014

Thank you for your reply.

I apologize for the confusion, there was a difference in the numerical order of amino acids listed in the alignment I was performing. You are correct, ab115883 should not be chosen as the immunogen sequence does not o...

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Thank you for contacting Abcam.

We discussed potential anti-Aurora A antibodies to detect Chlamydomonas reinhardtii Aurora A. Since C. reinhardtii Aurora A has 67.2% homology with human Aurora A, this does not qualify for our AbTrial program, ...

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A paper regarding use of the antibody in IP is Cremet JY et al., 2003 which is the last one on the
publication list on our datasheet for ab13824. http://www.ncbi.nlm.nih.gov/pubmed/12619897?dopt=Abstract
This also appears to be the licensors ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Primary keratinocytes)
Loading amount 20 µg
Specification Primary keratinocytes
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 26 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (SW620, IMR-32, 293, MCF7)
Loading amount 20 µg
Specification SW620, IMR-32, 293, MCF7
Gel Running Conditions Reduced Denaturing (12.5)
Blocking step 5% milk in PBS + 0.1% Tween as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Jun 24 2008

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab13824. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a pro...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Username

Dr. Kirk McManus

Verified customer

投稿 Apr 19 2007

1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"