製品の概要

  • 製品名
    Anti-AU5 tag antibody [AU5]
  • 製品の詳細
    Mouse monoclonal [AU5] to AU5 tag
  • アプリケーション
    適用あり: WB, ICC/IF, IPmore details
  • 免疫原

    This antibody was raised against an SDS disrupted BPV1 preparation and mapped to the six amino acid epitope TDFYLK.

  • エピトープ
    This antibody maps to the six amino acid epitope TDFYLK.
  • 特記事項


    This clone was specifically designed for use in epitope tagging. Because of its small size, clone AU5 is unlikely to alter the activity of the cloned sequence.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.03% Thimerosal (merthiolate)
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 一次抗体 備考
    This clone was specifically designed for use in epitope tagging. Because of its small size, clone AU5 is unlikely to alter the activity of the cloned sequence.
  • ポリ/モノ
    モノクローナル
  • クローン名
    AU5
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab24576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Predicted molecular weight: 1 kDa.
ICC/IF 1/500.
IP 1/150.

ターゲット情報

  • 関連性
    AU5 is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized. Because of the small size of the epitope, it is unlikely to alter the activity of the cloned sequence.
  • 別名
    • AU5 epitope tag antibody
    • TDFYLK epitope tag antibody
    • TDFYLK tag antibody

参考文献

ab24576 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Flow Cytometry
Sample
Mouse Cell (Yeast cell)
Permeabilization
No
Gating Strategy
Singlets of yeast
Specification
Yeast cell
Preparation
Cell harvesting/tissue preparation method: Activated in SG buffer
Sample buffer: PBSA
Fixation
None
Username

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投稿 Apr 25 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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