製品の概要

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/100 - 1/150. Primary: 1/1000 - 1/30000.
ICC 1/200 - 1/2000.
WB 1/1000 - 1/30000.
IP Use at an assay dependent dilution.

ターゲット情報

  • 別名
    • AU1 epitope tag antibody
    • DTYRYI epitope tag antibody
    • DTYRYI tag antibody

画像

  • The AU1 tag antibody (ab3401) was used in a Western blot to detect expression of mutant mammalian target of rapamycin (mTOR) proteins.  U373 and A172 human glioma cell lines were transfected with constructs encoding either an AU1 tagged rapamycin resistant (RR) mTOR or AU1 tagged kinase dead (KD) mTOR.  This detected the expression of a 290 kD protein corresponding to the AU1 tagged mutant mTOR proteins in both cell lines.  (The mutant mTOR proteins appear beneath a band corresponding to the wild-type (WT) mTOR protein - this was not detected by ab3401 but a different antibody).

    Picture from review by Amith Panner, submitted 29 July 2004.

    The AU1 tag antibody (ab3401) was used in a Western blot to detect expression of mutant mammalian target of rapamycin (mTOR) proteins. U373 and A172 human glioma cell lines were transfected with constructs encoding either an AU1 tagged rapamycin resistant (RR) mTOR or AU1 tagged kinase dead (KD) mTOR. This detected

参考文献

This product has been referenced in:
  • Chen G  et al. Bri2 BRICHOS client specificity and chaperone activity are governed by assembly state. Nat Commun 8:2081 (2017). Read more (PubMed: 29234026) »
  • Forstneric V  et al. The role of the C-terminal D0 domain of flagellin in activation of Toll like receptor 5. PLoS Pathog 13:e1006574 (2017). Read more (PubMed: 28827825) »

See all 8 Publications for this product

レビューと Q&A

Thank you for your enquiry and interest in our products.

This antibody has been tested in ICC so it is very likely to work in Confocal microscopy, though it has not been specifically characterized in this application.

If you need an...

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Je vous contacte suite aux questions auxquelles vous avez répondues il y a quelques jours concernant la qualité des produits et des services Abcam. Je vous remercie d'avoir pris le temps de répondre à ces questions.
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Merci de nous avoir contactés. Je suis désolé d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab3401. L'immunogène ayant servi à produire cet anticorps est DTYRYI. Je ne pense pas que la présence de 2 résidus avant cett...

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The AU1-AU5 tags are epitope tags and are fairly uncommon. In the instance of ab3401, the sequence DTYRYI is the sequence of the tag. If you have any more questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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