アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。
Our Abpromise guarantee covers the use of ab3401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/100 - 1/150. Primary: 1/1000 - 1/30000.|
|ICC||1/200 - 1/2000.|
|WB||1/1000 - 1/30000.|
|IP||Use at an assay dependent dilution.|
The AU1 tag antibody (ab3401) was used in a Western blot to detect expression of mutant mammalian target of rapamycin (mTOR) proteins. U373 and A172 human glioma cell lines were transfected with constructs encoding either an AU1 tagged rapamycin resistant (RR) mTOR or AU1 tagged kinase dead (KD) mTOR. This detected the expression of a 290 kD protein corresponding to the AU1 tagged mutant mTOR proteins in both cell lines. (The mutant mTOR proteins appear beneath a band corresponding to the wild-type (WT) mTOR protein - this was not detected by ab3401 but a different antibody).
Picture from review by Amith Panner, submitted 29 July 2004.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"