製品の概要

  • 製品名Anti-ATRX antibody
    ATRX 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ATRX
  • アプリケーション適用あり: IP, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Recombinant fragment corresponding to Human ATRX aa 2211-2413.
    Database link: P46100

  • ポジティブ・コントロール
    • 293T, HepG2, Raji, NIH-3T3, HeLa A431, H1299 and HeLaS3 cell lines. A549 xenograft.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 283 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

ターゲット情報

  • 機能Involved in transcriptional regulation and chromatin remodeling. Facilitates DNA replication in multiple cellular environments and is required for efficient replication of a subset of genomic loci. Binds to DNA tandem repeat sequences in both telomeres and euchromatin and in vitro binds DNA quadruplex structures. May help stabilizing G-rich regions into regular chromatin structures by remodeling G4 DNA and incorporating H3.3-containing nucleosomes. Catalytic component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Its heterochromatin targeting is proposed to involve a combinatorial readout of histone H3 modifications (specifically methylation states of H3K9 and H3K4) and association with CBX5. Involved in maintaining telomere structural integrity in embryonic stem cells which probably implies recruitment of CBX5 to telomers. Reports on the involvement in transcriptional regulation of telomeric repeat-containing RNA (TERRA) are conflicting; according to a report, it is not sufficient to decrease chromatin condensation at telomers nor to increase expression of telomeric RNA in fibroblasts (PubMed:24500201). May be involved in telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines. Acts as negative regulator of chromatin incorporation of transcriptionally repressive histone H2AFY, particularily at telomeres and the alpha-globin cluster in erythroleukemic cells. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, required for the chromatin occupancy of SMC1 and CTCTF within the H19 imprinting control region (ICR) and involved in esatblishment of histone tails modifications in the ICR. May be involved in brain development and facial morphogenesis. Binds to zinc-finger coding genes with atypical chromatin signatures and regulates its H3K9me3 levels. Forms a complex with ZNF274, TRIM28 and SETDB1 to facilitate the deposition and maintenance of H3K9me3 at the 3' exons of zinc-finger genes (PubMed:27029610).
  • 組織特異性Ubiquitous.
  • 関連疾患Alpha-thalassemia mental retardation syndrome, X-linked
    Mental retardation, X-linked, syndromic, with hypotonic facies 1
    Alpha-thalassemia myelodysplasia syndrome
  • 配列類似性Belongs to the SNF2/RAD54 helicase family.
    Contains 1 ADD domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 PHD-type zinc finger.
  • ドメインThe ADD domain predominantly interacts with histone H3 trimethylated at 'Lys-10'(H3K9me3) (and to a lesser extent H3 mono-or dimethylated at 'Lys-10') and simultanously to histone H3 unmethylated at 'Lys-5' (H3K4me0). The interaction with H3K9me3 is disrupted by the presence of H3K4me3 suggesting a readout of the combined histone H3 methylation state.
    Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
  • 翻訳後修飾Phosphorylated at serine residues during mitose. Phosphorylation may promote the release from the nuclear matrix and progression to mitosis.
  • 細胞内局在Nucleus. Chromosome, telomere. Nucleus, PML body. Associated with pericentromeric heterochromatin during interphase and mitosis, probably by interacting with CBX5/HP1 alpha. Colocalizes with histone H3.3, DAXX, HIRA and ASF1A at PML-nuclear bodies. Colocalizes with cohesin (SMC1 and SMC3) and MECP2 at the maternal H19 ICR (By similarity).
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha thalassemia/mental retardation syndrome X linked homolog antibody
    • ATP dependent helicase ATRX antibody
    • ATP-dependent helicase ATRX antibody
    • ATR2 antibody
    • Atrx antibody
    • ATRX_HUMAN antibody
    • DNA dependent ATPase and helicase antibody
    • Helicase 2, X linked antibody
    • MGC2094 antibody
    • MRXHF1 antibody
    • RAD54 antibody
    • RAD54L antibody
    • SFM1 antibody
    • SHS antibody
    • Transcriptional regulator ATRX antibody
    • X linked helicase II antibody
    • X linked nuclear protein antibody
    • X-linked helicase II antibody
    • X-linked nuclear protein antibody
    • XH2 antibody
    • XNP antibody
    • Znf HX antibody
    • Znf-HX antibody
    see all

Anti-ATRX antibody 画像

  • All lanes : Anti-ATRX antibody (ab97508) at 1/500 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 283 kDa
    5% SDS-PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97508 at 1/200 dilution. Lower image is merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma,using ab97508 at 1/500 dilution.

  • Anti-ATRX antibody (ab97508) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size : 283 kDa
    5% SDS-PAGE
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab97508 at 1/100 dilution.

Anti-ATRX antibody (ab97508) 使用論文

This product has been referenced in:
  • Valle-García D  et al. ATRX binds to atypical chromatin domains at the 3' exons of zinc finger genes to preserve H3K9me3 enrichment. Epigenetics N/A:0 (2016). ChIP, CHIPseq . Read more (PubMed: 27029610) »
  • Heckman LD  et al. Rett-causing mutations reveal two domains critical for MeCP2 function and for toxicity in MECP2 duplication syndrome mice. Elife 3:N/A (2014). ICC/IF ; Mouse . Read more (PubMed: 24970834) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - nuclear (K562 erythroleukemia cell line)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris gel)
Loading amount 10000 cells
Specification K562 erythroleukemia cell line
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. David Valle-Garcia

Verified customer

投稿 May 09 2016

Abreviews
Application ChIP
Sample Human Cell lysate - nuclear (K562 erythroleukemia cell line)
Negative control ZNF358 / ATRX KO cell line
Specification K562 erythroleukemia cell line
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control ZNF180, ZNF268, ZNF700, ZNF721
Username

Dr. David Valle-Garcia

Verified customer

投稿 Apr 14 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"