The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報IHC-P: 1/100 - 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
配列類似性Belongs to the ATRIP family.
ドメインThe EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
翻訳後修飾Phosphorylated by ATR.
細胞内局在Nucleus. Redistributes to discrete nuclear foci upon DNA damage.