製品の概要

  • 製品名Anti-ATRIP antibody
    ATRIP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ATRIP
  • 特異性ab59346 detects endogenous levels of total ATRIP protein.
  • アプリケーション適用あり: IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthesized non phosphopeptide derived from human ATRIP around the phosphorylation site of serine 68/serine 72 (LASPQA).

  • ポジティブ・コントロール
    • Human lung carcinoma tissue
  • 特記事項Store at -20°C for 1 year

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg+2 and Ca+2), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Affinity purified from rabbit antiserum by affinity chromatography, using epitope specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
WB 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).

ターゲット情報

  • 機能Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the ATRIP family.
  • ドメインThe EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
  • 翻訳後修飾Phosphorylated by ATR.
  • 細胞内局在Nucleus. Redistributes to discrete nuclear foci upon DNA damage.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AGS1 antibody
    • ATIP antibody
    • ATM and Rad3 related interacting protein antibody
    • ATM and Rad3-related-interacting protein antibody
    • ATR interacting protein antibody
    • ATR-interacting protein antibody
    • Atrip antibody
    • ATRIP_HUMAN antibody
    • DKFZp762J2115 antibody
    • FLJ12343 antibody
    • MGC20625 antibody
    • MGC21482 antibody
    • MGC26740 antibody
    see all

Anti-ATRIP antibody 画像

  • ab59346, at a 1/50 dilution, staining human ATRIP in lung carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence (left image) and presence of the immunizing peptide (right image).
  • Anti-ATRIP antibody (ab59346) at 1/1000 dilution + Human mammary cell lysate at 50000 cells

    Secondary
    Goat anti-rabbit HRP IgG at 1/2500 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 85 kDa
    Observed band size : 85 kDa


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-ATRIP antibody (ab59346) 使用論文

This product has been referenced in:
  • Neidhart M  et al. Trex-1 deficiency in rheumatoid arthritis synovial fibroblasts. Arthritis Rheum 62:2673-9 (2010). Flow Cyt ; Human . Read more (PubMed: 20496420) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human mammary cell)
Loading amount 50000 cells
Specification Human mammary cell
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Apr 17 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS
Username

Dr. Kirk McManus

Verified customer

投稿 Nov 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"