製品の概要

  • 製品名Anti-ATRIP antibody
    ATRIP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ATRIP
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    This information is considered to be commercially sensitive.

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab17962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/15000. Detects a band of approximately 95 kDa (predicted molecular weight: 86.8 kDa).

ターゲット情報

  • 機能Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the ATRIP family.
  • ドメインThe EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
  • 翻訳後修飾Phosphorylated by ATR.
  • 細胞内局在Nucleus. Redistributes to discrete nuclear foci upon DNA damage.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AGS1 antibody
    • ATIP antibody
    • ATM and Rad3 related interacting protein antibody
    • ATM and Rad3-related-interacting protein antibody
    • ATR interacting protein antibody
    • ATR-interacting protein antibody
    • Atrip antibody
    • ATRIP_HUMAN antibody
    • DKFZp762J2115 antibody
    • FLJ12343 antibody
    • MGC20625 antibody
    • MGC21482 antibody
    • MGC26740 antibody
    see all

Anti-ATRIP antibody 画像

  • Detection of Human ATRIP using ab17962 by Western Blot (2 x left images) and Immunoprecipitation (right image).

    Samples: Whole cell lysate (100 µg for WB; 400 µg for IP) from HeLa (A & B) and 293-T (A) cells.

    Antibodies: Affinity purified rabbit anti-ATRIP antibody (ab17962) used at 1/500 and 1/15000 dilution for WB and 3 µg/0.4 mg lysate for IP.

    ATRIP was also immunoprecipitated using a control antibody generated to recombinant human ATRIP:

    Right image - lane 1 ab17962; lane 2 control antibody; lane 3 normal IgG.

    Immunoprecipitated ATRIP was detected using the antibody to the recombinant protein. Detection: Chemiluminescence with exposure times of 5 minutes (2 x left) and 45 seconds (right image).

Anti-ATRIP antibody (ab17962) 使用論文

This product has been referenced in:
  • Sanz-García M  et al. Vaccinia-related Kinase 1 (VRK1) Is an Upstream Nucleosomal Kinase Required for the Assembly of 53BP1 Foci in Response to Ionizing Radiation-induced DNA Damage. J Biol Chem 287:23757-68 (2012). WB . Read more (PubMed: 22621922) »

See 1 Publication for this product

Product Wall

Thank you for your valuable feedback information. I have discussed this issue with my colleagues at Business Development Department and you are absolutely correct. We have already amended the on-line datasheet to prevent further confusion for our c...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"