製品の概要

  • 製品名Anti-ATR antibody
    ATR 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ATR
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Xenopus laevis
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human ATR and Rad3 related encoded within exon 29 (LocusLink ID 545).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa and U2OS cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: 1/15000 - 1/30000. Detects a band of approximately 300 kDa (predicted molecular weight: 307 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
    • 組織特異性Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
    • 関連疾患Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.
    • 配列類似性Belongs to the PI3/PI4-kinase family. ATM subfamily.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 2 HEAT repeats.
      Contains 1 PI3K/PI4K domain.
    • 翻訳後修飾Phosphorylated; autophosphorylates in vitro.
    • 細胞内局在Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
    • Information by UniProt
    • 参照データベース
      see all
    • 別名
      • Ataxia telangiectasia and Rad3 related antibody
      • Ataxia telangiectasia and Rad3-related protein antibody
      • ATR antibody
      • ATR_HUMAN antibody
      • FCTCS antibody
      • FRAP Related Protein 1 antibody
      • FRAP-related protein 1 antibody
      • FRP1 antibody
      • MEC1 antibody
      • MEC1 mitosis entry checkpoint 1 homolog antibody
      • Protein kinase ATR antibody
      • RAC3 antibody
      • Rad3 related protein antibody
      • SCKL antibody
      • SCKL1 antibody
      • Serine/threonine protein kinase ATR antibody
      • Serine/threonine-protein kinase ATR antibody
      see all

    Anti-ATR antibody 画像

    • All lanes : Anti-ATR antibody (ab10327) at 0.07 µg/ml

      Lane 1 : HeLa whole cell lysate
      Lane 2 : U2OS whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : U2OS whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Developed using the ECL technique

      Predicted band size : 307 kDa

    Anti-ATR antibody (ab10327) 使用論文

    This product has been referenced in:
    • Wang WH  et al. Hepatitis B virus X protein via the p38MAPK pathway induces E2F1 release and ATR kinase activation mediating p53 apoptosis. J Biol Chem 283:25455-67 (2008). WB ; Mouse . Read more (PubMed: 18606816) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. We recommend a 3-8% Tris-acetate gel. I will add the image of the blot of ATR in HeLa and U2OS cells on our datasheet as soon as possible to show the bands expected, Please do not hesitate to contact us again if you need...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"