The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.
Use a concentration of 1 µg/ml.
Use a concentration of 1 µg/ml. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Use a concentration of 1 µg/ml.
Use a concentration of 5 µg/ml.
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Isoform Heart is expressed specifically in the heart and skeletal muscle, which require rapid energy supply. Isoform Liver is expressed in the brain, liver and kidney. Isoform Heart and Isoform Liver are expressed in the skin, intestine, stomach and aorta.
ATP synthase subunit gamma, mitochondrial antibody
ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1 antibody
ATP synthase, H+ transporting, mitochondrial F1 complex, gamma subunit antibody
ATP5C 1 antibody
F ATPase gamma subunit antibody
F-ATPase gamma subunit antibody
Mitochondrial ATP synthase, gamma subunit 1 antibody
Western blot - Anti-ATPG antibody [2A1AA11] (ab119686)
All lanes : Anti-ATPG antibody [2A1AA11] (ab119686) at 1 µg/ml
Lane 1 : human heart homogenate lysate at 15 µg Lane 2 : human HepG2 cell lysate at 15 µg Lane 3 : human liver mitochondria lysate at 7.5 µg Lane 4 : rat liver mitochondria lysate at 7.5 µg Lane 5 : mouse liver mitochondria lysate at 7.5 µg Lane 6 : bovine heart mitochondria lysate at 7.5 µg
Secondary Goat anti-mouse HRP at 1/5000 dilution Developed using the ECL technique
Immunocytochemistry using ab119686 stained HDFn cells (human). The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min) with antigen retrieval. The cells were then incubated with the antibody (ab119686, 1µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondria.
IHC image of ATPG staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119686, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.