製品の概要

  • 製品名
  • 製品の詳細
    Chicken polyclonal to ATPB
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    The immunogen corresponds to C terminal amino acids 291-539 of Human Mitochondrial ATP Synthase Subunit Beta (ATPB).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab37922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 57 kDa.

ターゲット情報

  • 機能
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
  • 配列類似性
    Belongs to the ATPase alpha/beta chains family.
  • 細胞内局在
    Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATP 5B antibody
    • ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
    • ATP synthase subunit beta mitochondrial antibody
    • ATP synthase subunit beta, mitochondrial antibody
    • atp5b antibody
    • ATPB antibody
    • ATPB_HUMAN antibody
    • ATPMB antibody
    • ATPSB antibody
    • Epididymis secretory protein Li 271 antibody
    • HEL-S-271 antibody
    • Mitochondrial ATP synthase beta subunit antibody
    • Mitochondrial ATP Synthase Subunit Beta antibody
    • Mitochondrial ATP synthetase beta subunit antibody
    see all

画像

  • Anti-ATPB antibody (ab37922) at 1/2000 dilution + E coli-derived fusion protein at 2.5 µg

    Secondary
    Goat anti-IgY-HRP at 1/1000 dilution

    Predicted band size : 57 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab37922 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunocytochemistry
Sample
Human Cultured Cells (Podocytes)
Specification
Podocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Formaldehyde
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投稿 May 16 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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