製品の概要

  • 製品名Anti-ATPB antibody [4.3E8.D10]
    ATPB 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4.3E8.D10] to ATPB
  • 特異性Detects the beta subunit of ATP synthase (ATPB) from mouse rat and human samples. This antibody is useful as a mitochondrial marker.
  • アプリケーション適用あり: WB, ICC, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Other Immunogen Type corresponding to ATPB. Intact rat mitochondria.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ICC 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.
IP Use a concentration of 2 - 5 µg/ml.

By immunoprecipatation, this antibody detects an 50 kDa protein representing ATP synthase (ATPB) from solubilized rat brain mitochondria.

ターゲット情報

  • 機能Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
  • 配列類似性Belongs to the ATPase alpha/beta chains family.
  • 細胞内局在Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATP 5B antibody
    • ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
    • ATP synthase subunit beta mitochondrial antibody
    • ATP synthase subunit beta, mitochondrial antibody
    • atp5b antibody
    • ATPB antibody
    • ATPB_HUMAN antibody
    • ATPMB antibody
    • ATPSB antibody
    • Epididymis secretory protein Li 271 antibody
    • HEL-S-271 antibody
    • Mitochondrial ATP synthase beta subunit antibody
    • Mitochondrial ATP Synthase Subunit Beta antibody
    • Mitochondrial ATP synthetase beta subunit antibody
    see all

Anti-ATPB antibody [4.3E8.D10] 画像

  • Immunocytochemistry/Immunofluorescent analysis of ATPB (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with ab5432 at 1:100 overnight at 4ºC in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 microscope at 40X magnification.

  • Immunofluorescent analysis of ATPB in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATPB monoclonal antibody (ab5432) at a dilution of 1:200 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. ATPB staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunofluorescent analysis of ATPB in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATPB monoclonal antibody (ab5432) at a dilution of 1:200 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. ATPB staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunoprecipatation of rat neuronal/glial cell extract using ab5432.
  • ICC/IF image of ab5432 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunocytochemistry/Immunofluorescence analysis of rat neuronal/glial cell culture using ab5432.

  • Anti-ATPB antibody [4.3E8.D10] (ab5432) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 20 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 57 kDa


    Exposure time : 16 minutes

Anti-ATPB antibody [4.3E8.D10] (ab5432) 使用論文

This product has been referenced in:
  • Jans DC  et al. STED super-resolution microscopy reveals an array of MINOS clusters along human mitochondria. Proc Natl Acad Sci U S A 110:8936-41 (2013). Read more (PubMed: 23676277) »
  • Hilgendorf KI  et al. The retinoblastoma protein induces apoptosis directly at the mitochondria. Genes Dev 27:1003-15 (2013). WB . Read more (PubMed: 23618872) »

See all 8 Publications for this product

Product Wall

Thank you for your email. Yes, we do carry human mitochondrial lysates as well: Human Liver Mitochondrial lysate (ab110339) Human Heart Mitochondrial lysate (ab110337) However, both lysates come in WB loading buffer (ready to be used in WB) ...

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Merci pour votre appel de l'autre jour.

Je viens de recevoir la réponse du laboratoire. Malheureusement l'épitope du ab5432 n'as pas été déterminé non plus à notre connaissance.

Je su...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (head)
Loading amount 30 µg
Specification head
Gel Running Conditions Reduced Denaturing
Blocking step casein as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 4°C
Username

Ms. Zhiyin Xun

Verified customer

投稿 Aug 14 2007

Thank you for your enquiry and your patience. Based on the resources available for ATP Synthase Beta (ab5432), this product has not been epitope mapped yet. Occassionally customers will send us such information when they do the epitope mapping them...

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Thank you for your enquiry. The epitopes have not been determined for these two monoclonal antibodies. Ab5432 and ab14730 are different clones, and ab5432 was made using intact rat mitochondria as the immunogen, whereas human whole heart mitochondria w...

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Dr. Wang, Thank you for contacting us with your request. We do not have antibodies against all 13 of the proteins encoded by the mitochondrial genome yet. Right now we only seem to have an antibody against the COXI subunit, ab14705, which cross reac...

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Thank you for your enquiry. We are very sorry to hear you are having problem with this product. We regret to inform you that ab5432has not been tested for Western blot application only for IP and IF. Please could you take a look at the on-line datas...

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We haven't tested this antibody in WB applications (ab5432 has been tested in Immunoprecipitation and Immunofluorescence), therefore we don't know. Although, by immunoprecipatation, this antibody detects an 50 kDa protein representing ATP synthase fro...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"