製品の概要

  • 製品名Anti-ATP7b antibody [EPR6794]
    ATP7b 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6794] to ATP7b
  • アプリケーション適用あり: WB, Flow Cyt, ICC/IFmore details
    適用なし: IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues on the C-terminus of the Human ATP7B protein (P35670).

  • ポジティブ・コントロール
    • Caco-2 and HepG2 cell lysates and HepG2 cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 157 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • 追加情報Is unsuitable for IHC-P or IP.
  • ターゲット情報

    • 機能Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile.
    • 組織特異性Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues.
    • 関連疾患Defects in ATP7B are the cause of Wilson disease (WD) [MIM:277900]. WD is an autosomal recessive disorder of copper metabolism in which copper cannot be incorporated into ceruloplasmin in liver, and cannot be excreted from the liver into the bile. Copper accumulates in the liver and subsequently in the brain and kidney. The disease is characterized by neurologic manifestations and signs of cirrhosis.
    • 配列類似性Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.
      Contains 6 HMA domains.
    • 翻訳後修飾Isoform 1 may be proteolytically cleaved at the N-terminus to produce the WND/140 kDa form.
    • 細胞内局在Cytoplasm; Mitochondrion and Golgi apparatus > trans-Golgi network membrane. Predominantly found in the trans-Golgi network (TGN). Not redistributed to the plasma membrane in response to elevated copper levels.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ATP7B antibody
      • ATP7B_HUMAN antibody
      • ATPase, Cu(2+) transporting, beta polypeptide antibody
      • ATPase, Cu++ transporting, beta polypeptide antibody
      • Copper pump 2 antibody
      • Copper transporting ATPase 2 antibody
      • PWD antibody
      • Toxic milk antibody
      • tx antibody
      • WC1 antibody
      • WD antibody
      • Wilson disease associated protein antibody
      • Wilson disease-associated protein antibody
      • WND antibody
      • WND/140 kDa antibody
      see all

    Anti-ATP7b antibody [EPR6794] 画像

    • All lanes : Anti-ATP7b antibody [EPR6794] (ab124973) at 1/1000 dilution

      Lane 1 : Caco-2 cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 157 kDa
    • Flow cytometric analysis of permeabilized HepG2 cells using anti-ATP7B RabMAb in red (ab124973) or a rabbit IgG in green(negative).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-ATP7b antibody [EPR6794] (ab124973) 使用論文

    This product has been referenced in:

    See 1 Publication for this product

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