製品の概要

  • 製品名Anti-ATP7A antibody
    ATP7A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ATP7A
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cat, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to Human ATP7A. Short peptide sequence used to raise this antibody is 100% homologous to isoform 4 (1500aa, 163kDa), 1 (1514aa, 165kDa), 2 (1581aa, 172kDa), 5 (1422aa, 154kDa) of human ATP7A
    Database link: Q04656

  • ポジティブ・コントロール
    • WB: HepG2 cell lysate. IHC-P: Human kidney

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab42486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能May supply copper to copper-requiring proteins within the secretory pathway, when localized in the trans-Golgi network. Under conditions of elevated extracellular copper, it relocalized to the plasma membrane where it functions in the efflux of copper from cells.
  • 組織特異性Found in most tissues except liver. Isoform 3 is widely expressed including in liver cell lines. Isoform 1 is expressed in fibroblasts, choriocarcinoma, colon carcinoma and neuroblastoma cell lines. Isoform 2 is expressed in fibroblasts, colon carcinoma and neuroblastoma cell lines.
  • 関連疾患Menkes disease
    Occipital horn syndrome
    Distal spinal muscular atrophy, X-linked, 3
  • 配列類似性Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.
    Contains 6 HMA domains.
  • ドメインThe C-terminal di-leucine, 1487-Leu-Leu-1488, is an endocytic targeting signal which functions in retrieving recycling from the plasma membrane to the TGN. Mutation of the di-leucine signal results in the accumulation of the protein in the plasma membrane.
  • 細胞内局在Endoplasmic reticulum; Cytoplasm > cytosol and Golgi apparatus > trans-Golgi network membrane. Cell membrane. Cycles constitutively between the trans-Golgi network (TGN) and the plasma membrane. Predominantly found in the TGN and relocalized to the plasma membrane in response to elevated copper levels.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATP7A antibody
    • ATP7A_HUMAN antibody
    • Copper pump 1 antibody
    • Copper-transporting ATPase 1 antibody
    • DSMAX antibody
    • MC1 antibody
    • Menkes disease ATPase antibody
    • Menkes disease-associated protein antibody
    • Menkes syndrome antibody
    • MK antibody
    • MNK antibody
    • OHS antibody
    • SMAX3 antibody
    see all

Anti-ATP7A antibody 画像

  • Antibody ab42486 (4.0-8.0 ug/ml) immunohistochemistry staining of paraffin embedded human kidney. Positive staining of epithelial cells of human renal tubules idicated by arrows.Antibody ab42486 (4.0-8.0 ug/ml) immunohistochemistry staining of paraffin embedded human kidney. Positive staining of epithelial cells of human renal tubules idicated by arrows.
  • ICC/IF image of ab42486 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42486, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ATP7A antibody (ab42486) 使用論文

This product has been referenced in:
  • Anandhan A  et al. Overexpression of alpha-synuclein at non-toxic levels increases dopaminergic cell death induced by copper exposure via modulation of protein degradation pathways. Neurobiol Dis 81:76-92 (2015). WB ; Rat . Read more (PubMed: 25497688) »

See 1 Publication for this product

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We are still currently investigating the issue with ab42486. We are reviewingthe targetof this antibody and the datasheet will beupdated as soonas possible.

We don not have any information about the expression of ATP7A in muscle, ho...

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I have received your request for the replacement of ab42486with ab131400. Please note that ab131400 is not currently in stock and should be delivered to you within one week, maximum 2.

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It looks likewrong information was entered for ab42486. My colleaguesin charge of the product datasheets are reviewing this problem and the websitewill beupdated as soon a...

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Thank you very much for contacting us and for reporting this inconsistency. I just wanted to let you know that I am still working on your inquiry. I have contacted the originator of this product for the information you requested, and I am awaiting a...

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Thank you for your enquiry. I can confirm there are in two different isoforms for this protein, The 30 KD protein is a much shorter alternate isoform that is listed in both the NCBI an SwissProt databases and is sometimes detected for ATP7A. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"