Anti-ATP6V1G3 抗体 (ab122012)
Key features and details
- Rabbit polyclonal to ATP6V1G3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ATP6V1G3 antibody -
製品の詳細
Rabbit polyclonal to ATP6V1G3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
amino acids 36-115 (AKEEAMVEID QYRMQRDKEF RLKQSKIMGS QNNLSDEIEE QTLGKIQELN GHYNKYMESV MNQLLSMVCD MKPEIHVNYR) of Human ATP6V1G3 (Q96LB4).
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ポジティブ・コントロール
- WB: RT-4, U-251 MG, Human liver and tonsil lysate. IHC-P: Human kidney tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122012の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/250 - 1/500.
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IHC-P |
1/5000 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/250 - 1/500. |
IHC-P
1/5000 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. -
組織特異性
Kidney. -
配列類似性
Belongs to the V-ATPase G subunit family. - Information by UniProt
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参照データベース
- Entrez Gene: 127124 Human
- SwissProt: Q96LB4 Human
- Unigene: 127743 Human
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別名
- ATP6G3 antibody
- atp6v1g3 antibody
- ATPase, H+ transporting, lysosomal (vacuolar proton pump) subunit G3 antibody
see all
画像
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All lanes : Anti-ATP6V1G3 antibody (ab122012) at 1/250 dilution
Lane 1 : RT-4 lysate
Lane 2 : U-251 MG lysate
Lane 3 : Human plasma.
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique. -
Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human kidney tissue labeling ATP6V1G3 with ab122012 at 1/5000 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab122012 は 1 報の論文で使用されています。
- Heller EA et al. Locus-specific epigenetic remodeling controls addiction- and depression-related behaviors. Nat Neurosci 17:1720-7 (2014). PubMed: 25347353