製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ATP6V1B1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 395-444 (LMKSAIGEGM TRKDHGDVSN QLYACYAIGK DVQAMKAVVG EEALTSEDLL) of Human ATP6V1B1 (NP_001683).

  • ポジティブ・コントロール
    • HeLa cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab98991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
  • 組織特異性
    Expressed in the cochlea and endolymphatic sac.
  • 関連疾患
    Defects in ATP6V1B1 are the cause of distal renal tubular acidosis with deafness (dRTA-D) [MIM:267300]. Inheritance is autosomal recessive. Patients with recessive dRTA are severely affected, presenting with either acute illness or growth failure at a young age, and bilateral sensorineural deafness. Other features include low serum K(+) due to renal potassium wasting, and elevated urinary calcium. If untreated, this acidosis may result in dissolution of bone, leading to osteomalacia and rickets. Renal deposition of calcium salts (nephrocalcinosis) and renal stone formation commonly occur.
  • 配列類似性
    Belongs to the ATPase alpha/beta chains family.
  • ドメイン
    The PDZ-binding motif mediates interactions with SLC9A3R1 and SCL4A7.
  • 細胞内局在
    Endomembrane system. Endomembrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Atp6b1 antibody
    • ATP6V1B1 antibody
    • ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta 56/58 kDa, isoform 1 antibody
    • ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 1 antibody
    • ATPase, H+ transporting, V1 subunit B, isoform 1 antibody
    • Endomembrane proton pump 58 kDa subunit antibody
    • H(+) transporting two sector ATPase 58kD subunit antibody
    • H+ ATPase beta 1 subunit antibody
    • kidney isoform antibody
    • Lysosomal 56/58kDa antibody
    • MGC32642 antibody
    • RTA1B antibody
    • V ATPase B1 subunit antibody
    • V type proton ATPase subunit B kidney isoform antibody
    • V type proton ATPase subunit B, kidney isoform antibody
    • V-ATPase subunit B 1 antibody
    • V-type proton ATPase subunit B antibody
    • Vacuolar proton pump 3 antibody
    • Vacuolar proton pump subunit 3 antibody
    • Vacuolar proton pump subunit B 1 antibody
    • VATB antibody
    • VATB1_HUMAN antibody
    • VMA2 antibody
    • Vpp 3 antibody
    • Vpp3 antibody
    see all

Anti-ATP6V1B1 antibody 画像

  • Anti-ATP6V1B1 antibody (ab98991) at 1 µg/ml + HeLa cell lysate at 10 µg

    Predicted band size : 57 kDa

プロトコール

Anti-ATP6V1B1 antibody (ab98991) 使用論文

ab98991 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Aedes aegypti (Mosquito) Tissue lysate - whole (Renal tubule)
Specification
Renal tubule
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
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投稿 Oct 09 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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