製品の概要

  • 製品名Anti-ATP6V0D1 antibody
    ATP6V0D1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to ATP6V0D1
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: AKLFPHCGRL YPEGLAQLAR ADDYEQVKNV ADYYPEYKLL FEGAGSNPGD KTLEDRFFEH EVKLNKLAFL N, corresponding to amino acids 238-309 of Human ATP6V0D1

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab56441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis (By similarity). May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the V-ATPase V0D/AC39 subunit family.
  • 細胞内局在Membrane. Localizes to centrosome and the base of the cilium.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 32 kDa accessory protein antibody
    • ATP6D antibody
    • ATP6DV antibody
    • ATP6V0D1 antibody
    • ATPase H+ transporting lysosomal (vacuolar proton pump) member D antibody
    • ATPase H+ transporting lysosomal 38kD V0 subunit d antibody
    • ATPase H+ transporting lysosomal 38kDa V0 subunit d1 antibody
    • ATPase H+ transporting lysosomal V0 subunit d1 antibody
    • H(+) transporting two sector ATPase subunit D antibody
    • p39 antibody
    • V ATPase 40 KDa accessory protein antibody
    • V ATPase AC39 subunit antibody
    • V ATPase subunit d 1 antibody
    • V ATPase subunit D antibody
    • V-ATPase 40 kDa accessory protein antibody
    • V-ATPase AC39 subunit antibody
    • V-ATPase subunit d 1 antibody
    • V-type proton ATPase subunit d 1 antibody
    • VA0D1_HUMAN antibody
    • Vacuolar ATP synthase subunit d 1 antibody
    • Vacuolar proton pump subunit d 1 antibody
    • VATX antibody
    • VMA 6 antibody
    • VMA6 antibody
    • VPATPD antibody
    see all

Anti-ATP6V0D1 antibody 画像

  • ATP6V0D1 antibody (ab56441) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human stomach.


  • Predicted band size : 40 kDa
    ATP6V0D1 antibody (ab56441) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ICC/IF image of ab56441 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56441, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab56441 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56441, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was a mix of mouse IgG1 [ICIGG1], (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4%PFA/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-ATP6V0D1 antibody (ab56441) 使用論文

This product has been referenced in:
  • Matsuda S  et al. A cytotoxic type III secretion effector of Vibrio parahaemolyticus targets vacuolar H+-ATPase subunit c and ruptures host cell lysosomes. PLoS Pathog 8:e1002803 (2012). WB . Read more (PubMed: 22829766) »
  • Taguwa S  et al. Dysfunction of autophagy participates in vacuole formation and cell death in cells replicating hepatitis C virus. J Virol 85:13185-94 (2011). Read more (PubMed: 21994453) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Pig Cell lysate - other (LLC-PK1)
Gel Running Conditions Non-reduced Denaturing (4-15%)
Loading amount 10 µg
Specification LLC-PK1
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 21°C
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Dr. Christina McGuire

Verified customer

投稿 Dec 22 2015

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxxx.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"