製品の概要

  • 製品名Anti-ATP6V0C antibody
  • 製品の詳細
    Rabbit polyclonal to ATP6V0C
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Sheep, Rabbit, Horse, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 71-120 (VVAVLIANSL NDDISLYKSF LQLGAGLSVG LSGLAAGFAI GIVGDAGVRG) of Human ATP6V0C (NP_001685)

  • ポジティブ・コントロール
    • OVCAR3 cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab104374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 関連性ATP6V0C is a component of the V0 domain (proton pore complex) of vacuolar ATPase (V-ATPase). Vacuolar ATPase is a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.
  • 細胞内局在Vacuole membrane; Multi-pass membrane protein.
  • 参照データベース
  • 別名
    • ATP6C antibody
    • ATP6L antibody
    • ATPase, H+ transporting, lysosomal, V0 subunit c antibody
    • ATPL antibody
    • V ATPase subunit c antibody
    • Vacuolar ATP synthase 16 kDa proteolipid subunit antibody
    • Vacuolar H+ ATPase proton channel subunit antibody
    • Vacuolar proton pump, 16 kDa subunit antibody
    • VATL antibody
    • Vma3 antibody
    see all

Anti-ATP6V0C antibody 画像



  • Predicted band size : 16 kDa
    Western blot analysis of ACHN cell lysate labeling ATP6V0C with ab104374 at 1.0µg/ml.
  • ICC/IF image of ab104374 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104374 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-ATP6V0C antibody (ab104374) at 1 µg/ml + OVCAR3 cell lysate at 10 µg

    Predicted band size : 16 kDa
    Gel concentration 12%

Anti-ATP6V0C antibody (ab104374) 使用論文

ab104374 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab104374.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"