製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ATP6V0A4
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 53 and 265 of Human ATP6V0A4 (NP_570856)

  • ポジティブ・コントロール
    • A431, HeLa, HepG2, Molt-4 and Raji cell lines. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: Malme-3M

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 96 kDa.

ターゲット情報

  • 関連性
    ATP6V0A4 (ATPase, H+ transporting, lysosomal V0 subunit a isoform 4) is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain.
  • 細胞内局在
    Apical cell membrane; Multi-pass membrane protein.
  • 参照データベース
  • 別名
    • A4 antibody
    • ATP6N1B antibody
    • ATP6N2 antibody
    • ATPase, H+ transporting, lysosomal (vacuolar proton pump) noncatalytic accessory protein 2, 38kD antibody
    • ATPase, H+ transporting, lysosomal V0 subunit a4 antibody
    • ATPase, H+ TRANSPORTING, LYSOSOMAL, NONCATALYTIC ACCESSORY PROTEIN 1B antibody
    • MGC130016 antibody
    • MGC130017 antibody
    • RDRTA2 antibody
    • RTA1C antibody
    • RTADR antibody
    • STV1 antibody
    • V ATPase 116 kDa isoform a4 antibody
    • VACUOLAR PROTEIN PUMP, SUBUNIT 2 antibody
    • Vacuolar proton translocating ATPase 116 kDa subunit a isoform 4 antibody
    • Vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform antibody
    • VPH1 antibody
    • VPP2 antibody
    see all

Anti-ATP6V0A4 antibody 画像

  • ICC/IF image of ab97440 stained MALME-3M cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab97440 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-ATP6V0A4 antibody (ab97440) at 1/3000 dilution + Hep G2 whole cell lysate at 30 µg

    Predicted band size : 96 kDa
    7.5% SDS PAGE

Anti-ATP6V0A4 antibody (ab97440) 使用論文

ab97440 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney medulla)
Gel Running Conditions
Reduced Denaturing (NuPAGE 4-12% Bis-Tris)
Loading amount
7.5 µg
Specification
Kidney medulla
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Merkulova Maria

Verified customer

投稿 Mar 02 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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