製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ATP5J
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide mapping at the middle region of human mature ATP5J, different from the related mouse sequence by two amino acids.

  • ポジティブ・コントロール
    • Mammary cancer tissue and rat liver tissue lysate.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab65841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
  • 追加情報
    IHC-P: Use at a concentration of 1 - 2 µg/ml. Boiling the sections is required.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.
    • 配列類似性
      Belongs to the eukaryotic ATPase subunit F6 family.
    • 細胞内局在
      Mitochondrion. Mitochondrion inner membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 antibody
      • ATP synthase-coupling factor 6, mitochondrial antibody
      • ATP synthase-coupling factor 6, mitochondrial antibody
      • ATP5 antibody
      • ATP5A antibody
      • ATP5J antibody
      • ATP5J_HUMAN antibody
      • ATPase subunit F6 antibody
      • ATPM antibody
      • CF6 antibody
      • F6 antibody
      see all

    Anti-ATP5J antibody 画像

    • Anti-ATP5J antibody (ab65841) at 1 µg/ml + Rat liver tissue lysate

      Predicted band size : 13 kDa
      Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    • ab65841 at 2µg/ml staining ATP5J in Mammary cancer by Immunohistochemistry, Paraffin-embedded tissue (DAB chromogenic reaction).

    Anti-ATP5J antibody (ab65841) 使用論文

    This product has been referenced in:
    • Zeng Z  et al. Cyclic mechanical stretch promotes energy metabolism in osteoblast-like cells through an mTOR signaling-associated mechanism. Int J Mol Med 36:947-56 (2015). Read more (PubMed: 26251974) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab65841.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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