製品の概要

  • 製品名Anti-ATP5J antibody
    ATP5J 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ATP5J
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide mapping at the middle region of human mature ATP5J, different from the related mouse sequence by two amino acids.

  • ポジティブ・コントロール
    • Mammary cancer tissue and rat liver tissue lysate.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab65841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
  • 追加情報IHC-P: Use at a concentration of 1 - 2 µg/ml. Boiling the sections is required.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.
    • 配列類似性Belongs to the eukaryotic ATPase subunit F6 family.
    • 細胞内局在Mitochondrion. Mitochondrion inner membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 antibody
      • ATP synthase-coupling factor 6, mitochondrial antibody
      • ATP synthase-coupling factor 6, mitochondrial antibody
      • ATP5 antibody
      • ATP5A antibody
      • ATP5J antibody
      • ATP5J_HUMAN antibody
      • ATPase subunit F6 antibody
      • ATPM antibody
      • CF6 antibody
      • F6 antibody
      see all

    Anti-ATP5J antibody 画像

    • Anti-ATP5J antibody (ab65841) at 1 µg/ml + Rat liver tissue lysate

      Predicted band size : 13 kDa
      Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    • ab65841 at 2µg/ml staining ATP5J in Mammary cancer by Immunohistochemistry, Paraffin-embedded tissue (DAB chromogenic reaction).

    Anti-ATP5J antibody (ab65841) 使用論文

    ab65841 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"