製品の概要

  • 製品名Anti-ATP5F1 antibody [9D1BC4]
    ATP5F1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [9D1BC4] to ATP5F1
  • アプリケーション適用あり: IHC-P, WB, ICC, In-Cell ELISA, Flow Cyt, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Purified human liver mitochondria

  • ポジティブ・コントロール
    • Human normal colon FFPE tissue.
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab117991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 24.6 kDa.
ICC Use a concentration of 1 µg/ml.
In-Cell ELISA Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
  • 配列類似性Belongs to the eukaryotic ATPase B chain family.
  • 細胞内局在Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AT5F1_HUMAN antibody
    • ATP synthase B chain mitochondrial antibody
    • ATP synthase subunit b antibody
    • ATP synthase subunit b mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1 antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 antibody
    • ATP5F1 antibody
    • ATPase subunit b antibody
    • Cell proliferation inducing protein 47 antibody
    • H+ ATP synthase subunit b antibody
    • MGC24431 antibody
    • mitochondrial antibody
    • PIG47 antibody
    see all

Anti-ATP5F1 antibody [9D1BC4] 画像

  • ab117991 at 1 ug/ml with Hela cells in flow cytometry.
  • Immunocytochemistry image of ab117991 (MS972) stained NIH3T3 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min) with urea/heat antigen retrieval method. The cells were incubated with ab117991 at 1 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) AlexaFluor® 594goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). The target protein locates to the mitochondria. The four cells in the upper portion of the image show mitochondria in the elongated, reticular, arrangement, the three cells in the lower portion of the image show a punctuate mitochondrial organization and may be dividing/have recently divided.
  • All lanes : Anti-ATP5F1 antibody [9D1BC4] (ab117991) at 1 µg/ml

    Lane 1 : Human heart homogenate at 15 µg
    Lane 2 : HepG2 lysate at 15 µg
    Lane 3 : Human liver mitochondria at 7.5 µg
    Lane 4 : Bovine heart mitochondria at 7.5 µg
    Lane 5 : Rat liver mitochondria at 7.5 µg
    Lane 6 : Mouse liver mitochondria at 7.5 µg


    Predicted band size : 24.6 kDa
  • IHC image of ATP5F1 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117991, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-ATP5F1 antibody [9D1BC4] (ab117991) 使用論文

This product has been referenced in:
  • Milenkovic D  et al. TWINKLE is an essential mitochondrial helicase required for synthesis of nascent D-loop strands and complete mtDNA replication. Hum Mol Genet 22:1983-93 (2013). WB ; Mouse . Read more (PubMed: 23393161) »

See 1 Publication for this product

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Thank you for your question submitted during our ICC webinar last week. We hope you enjoyed the presentation and found this useful.

Our sister company, Mitosciences, often uses antigen retrieval with ICC testing of the antibodies we sell. I ...

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