Anti-ATP5E 抗体 (ab54879)

製品の概要

  • 製品名Anti-ATP5E antibody
  • 製品の詳細
    Mouse monoclonal to ATP5E
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein: MVAYWRQAGL SYIRYSQICA KAVRDALKTE FKANAEKTSG SNVKIVKVKK E, corresponding to amino acids 1-52 of Human ATP5E

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
  • 追加情報WB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
    • 組織特異性Ubiquitous.
    • 配列類似性Belongs to the eukaryotic ATPase epsilon family.
    • 細胞内局在Mitochondrion. Mitochondrion inner membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ATP 5E antibody
      • ATP synthase epsilon chain mitochondrial antibody
      • ATP synthase H+ transporting mitochondrial F1 complex epsilon subunit antibody
      • ATP synthase subunit epsilon antibody
      • ATP5E antibody
      • ATP5E_HUMAN antibody
      • ATPase subunit epsilon antibody
      • ATPE antibody
      • F(0)F(1) ATPase antibody
      • H(+) transporting two sector ATPase antibody
      • MGC104243 antibody
      • mitochondrial antibody
      • Mitochondrial ATP synthase epsilon chain antibody
      • Mitochondrial ATPase antibody
      see all

    Anti-ATP5E antibody 画像

    • ATP5E antibody (ab54879) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.


    • Predicted band size : 6 kDa
      ATP5E antibody (ab54879) at 1ug/lane + SW-13 cell lysate at 25ug/lane.

    Anti-ATP5E antibody (ab54879) 使用論文

    ab54879 has not yet been referenced specifically in any publications.

    Product Wall

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    You are right, it looks like the two products are made from the same clone. However, they differ in the concentration and purification method.

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    Application Western blot
    Sample Pig Tissue lysate - whole (myocardium)
    Loading amount 50 µg
    Specification myocardium
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Rahul Dalal

    Verified customer

    投稿 Sep 12 2012

    Thank you for contacting us.

    I am sorry that these two antibodies did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to con...

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    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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    I am very pleased to hear you would like to accept our offer and test ab54879 inpig. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for pigand include this code in the &ld...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"