製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ATP11B
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 144-193 (DIVRIAKDEI FPADLVLLSS DRLDGSCHVT TASLDGETNL KTHVAVPETA) of human ATP11B (NP_055431).

  • ポジティブ・コントロール
    • Fetal liver lysate (human)

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified by peptide affinity chromatography method.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 134 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

画像

  • Anti-ATP11B antibody (ab82640) at 1 µg/ml + human fetal liver lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 134 kDa
    Observed band size: 144 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab82640 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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