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ab109716 rapid multiplexing microplate kit is used to determine both the activity and quantity of ATP synthase (Complex V) in a Human or Rat sample. The ratio of the two measurements represents the enzyme's specific activity. The ATP synthase enzyme is immunocaptured within the wells of the microplate and the enzyme activity is measured by monitoring the decrease in absorbance at 340 nm. Specifically, the conversion of ATP to ADP by ATP synthase is coupled to the oxidative reaction of NADH to NAD+. The formation of NAD+ results in a decrease in absorbance at 340 nm. Subsequently, in these same wells, the quantity of ATP synthase is determined by adding an ATP synthase specific antibody conjugated with alkaline phosphatase. This phosphatase changes the substrate pNPP from colorless to yellow (OD 405 nm) in a time dependent manner proportional to the amount of protein captured in the wells. Included for optimal performance of the assay are buffer, detergent, antibody, substrate, lipid mix and a 96-well microplate pre-coated with monoclonal antibody.
Store Buffer, Detergent, Detector Antibody, AP Label, AP Buffer, and the microplate at 4°C. Store the Reagent Mix, AP Development Reagent and Lipid Mix at -20°C. For multiple experiments, the Reagent Mix and AP Development Reagent may be aliquoted and stored at -20°C, or at -80°C for long term storage.
|AP ATP synthase Detector antibody||1 x 1ml|
|AP Buffer||Tube 2||1 x 20ml|
|AP Development Reagent||1 x 400µl|
|AP label 2500 X||Tube B||1 x 10µl|
|ATP synthase precoated Microtiter plate||1 unit|
|Buffer||Tube 1||1 x 15ml|
|Detergent||1 x 1ml|
|Phospholipid||1 x 6ml|
|Reagent Mix||1 x 20ml|
Our Abpromise guarantee covers the use of ab109716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|