Anti-ATP Synthase beta 抗体 (ab43176)

製品の概要

  • 製品名Anti-ATP Synthase beta antibody
  • 製品の詳細
    Chicken polyclonal to ATP Synthase beta
  • 特異性This antibody will detect the mitochondrial form of the F1 ATP Synthase subcomplex, as well as the chloroplastic CF1 Atp Synthase, and most known bacterial F-type Atp Synthases. This antibody will not cross-react with -V-type ATP Synthase, found mainly in Archaea.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    Chloroplasts, mitochondria and most bacteria.
  • 免疫原

    Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the ATP Synthase beta protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab43176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: 1/5000 - 1/50000. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).

    Immunolocalization: Use at an assay-dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Mitochondrial membrane ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk.
    • 細胞内局在Mitochondrion. Mitochondrion inner membrane. Note=Peripheral membrane protein.
    • 別名
      • ATP synthase beta chain 1, mitochondrial antibody
      • ATP synthase beta chain 2, mitochondrial antibody

    Anti-ATP Synthase beta antibody 画像

    • All lanes : Anti-ATP Synthase beta antibody (ab43176) at 1/5000 dilution

      Lane 1 : Markers 20, 30, 40, 50, 60, 80, 100, 120, 220kDa.
      Lane 2 : Extract of Synechocystis
      Lane 3 : Isolated thylakoid fraction of Spinach

      Secondary
      HRP-conjugated anti IgY, from Abcam at 1/50000 dilution
      Developed using the ECL technique

      Predicted band size : 59 kDa
      Observed band size : 52,54 kDa (why is the actual band size different from the predicted?)

    • Developed using the ECL technique

      Predicted band size : 59 kDa


      Exposure time : 10 seconds

      Lanes 1-3: ATP Synthase beta protein standard 0.03, 0.1, 0.26 pmol. Lanes 4-8: Total protein from Trichodesmium IMS 101 extracted with PEB. Samples were separated on 4-12% SDS-PAGE and blotted for 1 hour to PVDF and blocked immediately following transfer in 2% ECL blocking reagent in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1 hour at room temperature with agitation. Blots were incubated in the primary antibody (ab43176) at 1/50000 for 1 hour at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 minutes and 3 times for 5 minutes in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (HRP-conjugated anti-IgY) diluted to 1/50000 in 2% ECL blocking solution for 1 hour at room temperature with agitation. The blots were washed as above and developed for 5 minutes with ECL detection reagent. Exposure time was 10 seconds.

    Anti-ATP Synthase beta antibody (ab43176) 使用論文

    ab43176 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"