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Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the ATP Synthase beta protein.
Our Abpromise guarantee covers the use of ab43176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Lanes 1-3: ATP Synthase beta protein standard 0.03, 0.1, 0.26 pmol. Lanes 4-8: Total protein from Trichodesmium IMS 101 extracted with PEB. Samples were separated on 4-12% SDS-PAGE and blotted for 1 hour to PVDF and blocked immediately following transfer in 2% ECL blocking reagent in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1 hour at room temperature with agitation. Blots were incubated in the primary antibody (ab43176) at 1/50000 for 1 hour at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 minutes and 3 times for 5 minutes in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (HRP-conjugated anti-IgY) diluted to 1/50000 in 2% ECL blocking solution for 1 hour at room temperature with agitation. The blots were washed as above and developed for 5 minutes with ECL detection reagent. Exposure time was 10 seconds.
ab43176 has not yet been referenced specifically in any publications.
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