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RabMAb

Anti-ATP citrate lyase (phospho T447 + S451) 抗体 [EP737Y] (ab53007)

製品の概要

  • 製品名
    Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y]
    ATP citrate lyase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP737Y] to ATP citrate lyase (phospho T447 + S451)
  • 特異性
    ab53007 only detects ATP-citrate lyase phosphorylated on Threonine 447 and Serine 451.
  • アプリケーション
    適用あり: WB, ICC/IF, IPmore details
    適用なし: Flow Cyt or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Thr447 and Ser451 of human ATP-citrate (pro-S) lyase.

  • ポジティブ・コントロール
    • 3T3 cytoplasmic cell lysate HeLa cells
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab53007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 122 kDa).
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • 追加情報
    Is unsuitable for Flow Cyt or IHC-P.
  • ターゲット情報

    • 機能
      ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
    • 配列類似性
      In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
      In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
      Contains 1 ATP-grasp domain.
    • 翻訳後修飾
      ISGylated.
      Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
      Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ACL antibody
      • Acly antibody
      • ACLY_HUMAN antibody
      • ATP citrate (pro-S) lyase antibody
      • ATP citrate lyase antibody
      • ATP citrate synthase antibody
      • ATP-citrate (pro-S-)-lyase antibody
      • ATP-citrate synthase antibody
      • ATPcitrate synthase antibody
      • ATPCL antibody
      • Citrate cleavage enzyme antibody
      • CLATP antibody
      • OTTHUMP00000164773 antibody
      see all

    Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] 画像

    • All lanes : Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) at 1/1000 dilution

      Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) treated with insulin whole cell lysates.
      Lane 2 : Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysates.

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated. at 1/2000 dilution

      Predicted band size : 122 kDa


      Exposure time : 3 minutes

      Blocking and diluting buffer: 5% NFDM/TBST

    • ab53007 stained HeLa cells at 1/100-1/250 dilution.

    Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) 使用論文

    This product has been referenced in:
    • Grunert T  et al. A comparative proteome analysis links tyrosine kinase 2 (Tyk2) to the regulation of cellular glucose and lipid metabolism in response to poly(I:C). J Proteomics 74:2866-80 (2011). WB . Read more (PubMed: 21787891) »
    • Chu KY  et al. ATP-citrate lyase reduction mediates palmitate-induced apoptosis in pancreatic beta cells. J Biol Chem 285:32606-15 (2010). Read more (PubMed: 20693577) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mouse ESCs)
    Permeabilization
    Yes - TritonX
    Specification
    mouse ESCs
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 37°C
    Fixative
    Formaldehyde
    Username

    Diego Rodriguez Terrones

    Verified customer

    投稿 Sep 01 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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