Anti-ATP citrate lyase (phospho S455) 抗体 [EP828Y] (ab46796)

製品の概要

  • 製品名
    Anti-ATP citrate lyase (phospho S455) antibody [EP828Y]
    ATP citrate lyase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP828Y] to ATP citrate lyase (phospho S455)
  • アプリケーション
    適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues of ATP citrate lyase (Human).

  • ポジティブ・コントロール
    • Insulin treated 3T3 cell lysate.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab46796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000. Detects a band of approximately 125 kDa.
IP 1/20.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • ターゲット情報

    • 機能
      ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
    • 配列類似性
      In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
      In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
      Contains 1 ATP-grasp domain.
    • 翻訳後修飾
      ISGylated.
      Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
      Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ACL antibody
      • Acly antibody
      • ACLY_HUMAN antibody
      • ATP citrate (pro-S) lyase antibody
      • ATP citrate lyase antibody
      • ATP citrate synthase antibody
      • ATP-citrate (pro-S-)-lyase antibody
      • ATP-citrate synthase antibody
      • ATPcitrate synthase antibody
      • ATPCL antibody
      • Citrate cleavage enzyme antibody
      • CLATP antibody
      • OTTHUMP00000164773 antibody
      see all

    画像

    • All lanes : Anti-ATP citrate lyase (phospho S455) antibody [EP828Y] (ab46796) at 1/5000 dilution

      Lane 1 : Untreated 3T3 cell lysates
      Lane 2 : Insulin treated 3T3 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Observed band size : 125 kDa (why is the actual band size different from the predicted?)

    参考文献

    ab46796 has not yet been referenced specifically in any publications.

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