Anti-ATP citrate lyase 抗体 [3G8] (ab118150)

製品の概要

  • 製品名Anti-ATP citrate lyase antibody [3G8]
    ATP citrate lyase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3G8] to ATP citrate lyase
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length Human ATP citrate lyase produced in HEK293T cells (NP_001087).

  • ポジティブ・コントロール
    • HEK293T cell lysate transfected with pCMV6-ENTRY ATP citrate lyase transfected; Human breast adenocarcinoma, colon, colon adenocarcinoma, kidney, liver, liver carcinoma, lung, ovary adenocarcinoma, pancreas, pancreas carcinoma, thyroid carcinoma, endometrium, endometrium adenocarcinoma, prostate, prostate carcinoma, and bladder tissies; COS7 cells transiently transfected by pCMV6-ENTRY ATP citrate lyase.
  • 特記事項Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab118150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 120 kDa.
IHC-P 1/50.
ICC/IF 1/100.

ターゲット情報

  • 機能ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
  • 配列類似性In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
    In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
    Contains 1 ATP-grasp domain.
  • 翻訳後修飾ISGylated.
    Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
    Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACL antibody
    • Acly antibody
    • ACLY_HUMAN antibody
    • ATP citrate (pro-S) lyase antibody
    • ATP citrate lyase antibody
    • ATP citrate synthase antibody
    • ATP-citrate (pro-S-)-lyase antibody
    • ATP-citrate synthase antibody
    • ATPcitrate synthase antibody
    • ATPCL antibody
    • Citrate cleavage enzyme antibody
    • CLATP antibody
    • OTTHUMP00000164773 antibody
    see all

Anti-ATP citrate lyase antibody [3G8] 画像

  • All lanes : Anti-ATP citrate lyase antibody [3G8] (ab118150) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY ATP citrate lyase cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 120 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200508)
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human breast adenocarcinoma tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human colon tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human liver tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human liver carcinoma tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human lung tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human pancreas tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human pancreas carcinoma tissue by Immunohistochemistry..
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human thyroid tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human endometrium tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human prostate tissue by Immunohistochemistry.
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry..
  • ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human bladder tissue by Immunohistochemistry.
  • Immunofluorescent staining of COS7 cells transiently transfected by ATP citrate lyase using ab118150 at a dilution of 1/100.

Anti-ATP citrate lyase antibody [3G8] (ab118150) 使用論文

ab118150 has not yet been referenced specifically in any publications.

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