製品の概要

  • 製品名Anti-ATM antibody [ATM 11G12]
    ATM 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [ATM 11G12] to ATM
  • アプリケーション適用あり: ICC/IF, Flow Cyt, WB, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 992-1144 of ATM

  • ポジティブ・コントロール
    • Lymphoblastoid cell line lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. See Abreview.
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/500. Predicted molecular weight: 350 kDa.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends.
  • 組織特異性Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.
  • 関連疾患Defects in ATM are the cause of ataxia telangiectasia (AT) [MIM:208900]; also known as Louis-Bar syndrome, which includes four complementation groups: A, C, D and E. This rare recessive disorder is characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. AT patients have a strong predisposition to cancer; about 30% of patients develop tumors, particularly lymphomas and leukemias. Cells from affected individuals are highly sensitive to damage by ionizing radiation and resistant to inhibition of DNA synthesis following irradiation.
    Note=Defects in ATM contribute to T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL). TPLL is characterized by a high white blood cell count, with a predominance of prolymphocytes, marked splenomegaly, lymphadenopathy, skin lesions and serous effusion. The clinical course is highly aggressive, with poor response to chemotherapy and short survival time. TPLL occurs both in adults as a sporadic disease and in younger AT patients.
    Note=Defects in ATM contribute to B-cell non-Hodgkin lymphomas (BNHL), including mantle cell lymphoma (MCL).
    Note=Defects in ATM contribute to B-cell chronic lymphocytic leukemia (BCLL). BCLL is the commonest form of leukemia in the elderly. It is characterized by the accumulation of mature CD5+ B lymphocytes, lymphadenopathy, immunodeficiency and bone marrow failure.
  • 配列類似性Belongs to the PI3/PI4-kinase family. ATM subfamily.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 1 PI3K/PI4K domain.
  • ドメインThe FATC domain is required for interaction with KAT5.
  • 翻訳後修飾Phosphorylated by NUAK1/ARK5. Autophosphorylation on Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-mediated activation of the kinase.
    Acetylation, on DNA damage, is required for activation of the kinase activity, dimer-monomer transition, and subsequent autophosphorylation on Ser-1981. Acetylated in vitro by KAT5/TIP60.
  • 細胞内局在Nucleus. Cytoplasmic vesicle. Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A-T mutated antibody
    • A-T mutated homolog antibody
    • AT mutated antibody
    • AT1 antibody
    • ATA antibody
    • Ataxia telangiectasia mutated antibody
    • Ataxia telangiectasia mutated gene antibody
    • Ataxia telangiectasia mutated homolog (human) antibody
    • Ataxia telangiectasia mutated homolog antibody
    • ATC antibody
    • ATD antibody
    • ATDC antibody
    • ATE antibody
    • ATM antibody
    • ATM serine/threonine kinase antibody
    • ATM_HUMAN antibody
    • DKFZp781A0353 antibody
    • MGC74674 antibody
    • OTTHUMP00000232981 antibody
    • Serine protein kinase ATM antibody
    • Serine-protein kinase ATM antibody
    • Serine/threonine-protein kinase ATM antibody
    • Tefu antibody
    • TEL1 antibody
    • TEL1, telomere maintenance 1, homolog antibody
    • TELO1 antibody
    • Telomere fusion protein antibody
    see all

Anti-ATM antibody [ATM 11G12] 画像

  • All lanes : Anti-ATM antibody [ATM 11G12] (ab31842) at 1/500 dilution

    Lane 1 : Lysate of lymphoblastoid cell line from a normal patient showing ATM
    Lane 2 : Lysate from a classical A-T patient with two truncating ATM mutations showing no ATM protein expression
    Lane 3 : Lysate from an A-T patient carrying a splicing mutation that results in expression of ~5% of normal ATM
    Lane 4 : Lysate of lymphoblastoid cell line from a normal patient showing ATM


    Predicted band size : 350 kDa
  • Overlay histogram showing HeLa cells stained with ab31842 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab31842, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-ATM antibody [ATM 11G12] (ab31842) 使用論文

This product has been referenced in:
  • Tian K  et al. Dynamics of DNA damage induced pathways to cancer. PLoS One 8:e72303 (2013). WB . Read more (PubMed: 24023735) »
  • Reiman A  et al. Lymphoid tumours and breast cancer in ataxia telangiectasia; substantial protective effect of residual ATM kinase activity against childhood tumours. Br J Cancer 105:586-91 (2011). WB ; Human . Read more (PubMed: 21792198) »

See all 4 Publications for this product

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Wie am Telefon besprochen, ist unseres Wissens nach der Antikörper ab31842bisher noch nicht inImmunohistochemie (IHC)getestet worden. Falls Sie dies selbs...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa and SK-N-SH)
Specification HeLa and SK-N-SH
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

投稿 Oct 04 2006

Thank you for your enquiry and patience. I heard back from our source for this antibody, and unfortunately we don't have information regarding exactly how much protein was loaded onto the gel for the image shown. We normally recommend loading at least ...

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