製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ATL3
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Hamster, Human
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 230-260 of Human ATL3, conjugated to KLH.

  • ポジティブ・コントロール
    • Mouse liver tissue lysates and CHO cell line lysates This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC3.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab104262 is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab104262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 23151802
WB 1/100 - 1/500. Predicted molecular weight: 61 kDa.

ターゲット情報

  • 機能
    GTPase tethering membranes through formation of trans-homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis.
  • 組織特異性
    Expressed in peripheral tissues (at protein level).
  • 配列類似性
    Belongs to the GBP family. Atlastin subfamily.
  • 細胞内局在
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4633402C03Rik antibody
    • 5730596K20Rik antibody
    • AI465397 antibody
    • ATL3 antibody
    • ATLA3_HUMAN antibody
    • Atlastin 3 like antibody
    • Atlastin GTPase 3 antibody
    • Atlastin-3 antibody
    • AW228836 antibody
    • DKFZp564J0863 antibody
    • MGC28761 antibody
    see all

画像

  • ICC/IF image of ab104262 stained PC3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104262 at 0.2µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-ATL3 antibody (ab104262) at 1/100 dilution + Mouse liver tissue lysate at 35 µg

    Predicted band size : 61 kDa
  • Anti-ATL3 antibody (ab104262) at 1/100 dilution + CHO cell lysate at 35 µg

    Predicted band size : 61 kDa

参考文献

This product has been referenced in:
  • Yang YM  et al. Deletion of STAT5a/b in vascular smooth muscle abrogates the male bias in hypoxic pulmonary hypertension in mice: implications in the human disease. Mol Med 20:625-38 (2015). Mouse . Read more (PubMed: 25470773) »
  • Lee JE  et al. Definitive evidence using enucleated cytoplasts for a nongenomic basis for the cystic change in endoplasmic reticulum structure caused by STAT5a/b siRNAs. Am J Physiol Cell Physiol 304:C312-23 (2013). WB, ICC/IF ; Human . Read more (PubMed: 23151802) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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