The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
追加情報Is unsuitable for WB.
機能Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
組織特異性Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
Detection of ATG4B by Western Blot of Immunprecipitate.
An antibody to ATG4B, at 1 µg/ml, staining ATG4B in HeLa whole cell lysate immunoprecipitated using ab101983 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 30 seconds.
Anti-ATG4B antibody (ab101983) 使用論文
has not yet been referenced specifically in any publications.