製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ATG2B
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 117-146 of Human ATG2B (NP_060506.5).

  • ポジティブ・コントロール
    • CEM cell line lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab116215 is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab116215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 233 kDa.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 配列類似性
    Belongs to the ATG2 family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2410024A21Rik antibody
    • AI047755 antibody
    • AI503411 antibody
    • ATG2 autophagy related 2 homolog B antibody
    • Atg2b antibody
    • ATG2B_HUMAN antibody
    • Autophagy-related protein 2 homolog B antibody
    • AW558123 antibody
    • C030004M05Rik antibody
    • C14orf103 antibody
    • C630028L02Rik antibody
    • FLJ10242 antibody
    • KIAA4067 antibody
    • mKIAA4067 antibody
    see all

画像

  • Anti-ATG2B antibody (ab116215) at 1/100 dilution + CEM cell line lysate at 35 µg

    Predicted band size : 233 kDa
  • ab116215 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab116215 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab116215 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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