製品の概要

  • 製品名Anti-ATF7 antibody
    ATF7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ATF7
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region within the C terminal amino acids 444-494 (ATAPSNGLSV RSAAEAVATS VLTQMASQRT ELSMPIQSHV IMTPQSQSAG R) of human ATF7 (CAA40483.1).

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 10 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 53 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family.
      Isoform 4/ATF-A0 acts as a dominant repressor of the E-selectin/NF-ELAM1/delta-A promoter.
    • 組織特異性Expressed in heart, lung and skeletal muscle. Isoform 4 is expressed in various tissues including heart, brain, placenta, lung and skeletal muscle. Highest levels in skeletal muscle. Lowest in lung and placenta.
    • 配列類似性Belongs to the bZIP family.
      Contains 1 bZIP domain.
      Contains 1 C2H2-type zinc finger.
    • 翻訳後修飾On EGF stimulation, phosphorylated first on Thr-53 allowing subsequent phosphorylation on Thr-51. This latter phosphorylation prevents sumoylation, increases binding to TAF12 and enhances transcriptional activity.
      Sumoylation delays nuclear localization and inhibits transactivation activity through preventing binding to TAF12. RANBP2 appears to be the specific E3 ligase.
    • 細胞内局在Nucleus. Nucleus > nucleoplasm. Mainly nucleoplasmic. Restricted distribution to the perinuculear region. The sumoylated form locates to the nuclear periphery.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 1110012F10Rik antibody
      • 9430065F09Rik antibody
      • Activating transcription factor 7 antibody
      • AI549878 antibody
      • ATF7 antibody
      • Atf7 protein antibody
      • ATF7_HUMAN antibody
      • ATFA antibody
      • C130020M04Rik antibody
      • cAMP-dependent transcription factor ATF-7 antibody
      • Cyclic AMP-dependent transcription factor ATF 7 antibody
      • Cyclic AMP-dependent transcription factor ATF-7 antibody
      • MGC31554 antibody
      • MGC57182 antibody
      • Transcription factor ATF A antibody
      • Transcription factor ATF-A antibody
      see all

    Anti-ATF7 antibody 画像

    • All lanes : Anti-ATF7 antibody (ab87844) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 53 kDa
      Observed band size : 63 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds
    • Detection of human ATF7 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab87844 at 10 µg/mg lysate for IP. Subsequent Western blot detection used ab87844 at 1 µg/ml.
      Developed using the ECL technique, with an exposure time of 10 seconds.

    Anti-ATF7 antibody (ab87844) 使用論文

    ab87844 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab87844.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"