製品の概要

  • 製品名Anti-ATF5 antibody
    ATF5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ATF5
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Chicken, Cow
  • 免疫原

    Synthetic peptide (Human)

  • ポジティブ・コントロール
    • Jurkat cell lysate.
  • 特記事項MASLLKKELEQMEDFFLDAPPLPPPSPPPLPPPPLPPAPSLPLSLPSFDL sequence information

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab60126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 関連性ATF5 or Activating transcription factor 5, binds to cAMP inducible promoters and is involved in gene transcription. This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. ATF5 plays a role in inhibition of nerve growth factor induced neuronal outgrowth and regulation of neurogenesis.
  • 細胞内局在Cytoplasmic and Nuclear
  • 参照データベース
  • 別名
    • Activating transcription factor 5 alpha/beta antibody
    • Activating transcription factor 5 antibody
    • Activating transcription factor X antibody
    • AFTA antibody
    • ATF 5 antibody
    • ATF 7 antibody
    • ATF7 antibody
    • ATFX antibody
    • BZIP protein ATF7 antibody
    • cAMP dependent transcription factor ATF 5 antibody
    • cAMP dependent transcription factor ATF5 antibody
    • Cyclic AMP dependent transcription factor ATF 5 antibody
    • Cyclic AMP dependent transcription factor ATF5 antibody
    • FLJ34666 antibody
    • HMFN0395 antibody
    • MGC102397 antibody
    • NAP1 antibody
    • NRIF3 associated protein antibody
    • ODA 10 antibody
    • Transcription factor ATFx antibody
    • Transcription factor like protein ODA 10 antibody
    • Transcription factor like protein ODA10 antibody
    see all

Anti-ATF5 antibody 画像

  • Anti-ATF5 antibody (ab60126) at 0.25 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/ 100,000 dilution.

    Predicted band size : 31 kDa
    Observed band size : 31 kDa
  • ICC/IF image of ab60126 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60126, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab60126 staining in human normal lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab60126, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry of Liver tissue at an antibody concentration of 4-8µg/ml using anti-ATF5 antibody (ab60126)

Anti-ATF5 antibody (ab60126) 使用論文

This product has been referenced in:
  • Liu X  et al. Nucleophosmin (NPM1/B23) Interacts with Activating Transcription Factor 5 (ATF5) Protein and Promotes Proteasome- and Caspase-dependent ATF5 Degradation in Hepatocellular Carcinoma Cells. J Biol Chem 287:19599-609 (2012). WB . Read more (PubMed: 22528486) »
  • Dluzen D  et al. BCL-2 is a downstream target of ATF5 that mediates the prosurvival function of ATF5 in a cell type-dependent manner. J Biol Chem 286:7705-13 (2011). WB . Read more (PubMed: 21212266) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"