Anti-ATF2 (phospho T71) 抗体 [E268] (ab32019)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E268] to ATF2 (phospho T71)
- Suitable for: WB, IP, ICC/IF, Dot blot
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ATF2 (phospho T71) antibody [E268]
ATF2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [E268] to ATF2 (phospho T71) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICC/IF, Dot blotmore details
適用なし: Flow Cyt or IHC -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human ATF2 aa 50-150.
Database link: P15336 -
ポジティブ・コントロール
- IP: HeLa treated with 250ng/ml anisomycin for 30min whole cell lysate. ICC/IF: HeLa cells treated with 250ng/ml anisomycin for 30min.
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特記事項
SAPK and p38 MAPK activate, in response to cellular stress, ATF2 by phosphorylating the protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress induced transcription by ATF2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
一次抗体 備考
SAPK and p38 MAPK activate, in response to cellular stress, ATF2 by phosphorylating the protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress induced transcription by ATF2. -
ポリ/モノ
モノクローナル -
クローン名
E268 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32019の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 54 kDa).
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IP |
1/30.
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ICC/IF |
1/500.
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Dot blot |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 54 kDa). |
IP
1/30. |
ICC/IF
1/500. |
Dot blot
Use at an assay dependent concentration. |
ターゲット情報
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機能
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex. -
組織特異性
Abundant expression seen in the brain. -
配列類似性
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger. -
翻訳後修飾
Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1386 Human
- Entrez Gene: 100047997 Mouse
- Entrez Gene: 11909 Mouse
- Omim: 123811 Human
- SwissProt: P15336 Human
- SwissProt: P16951 Mouse
- Unigene: 592510 Human
- Unigene: 209903 Mouse
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別名
- Activating transcription factor 2 antibody
- Activating transcription factor 2 splice variant ATF2 var2 antibody
- ATF 2 antibody
see all
画像
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All lanes : Anti-ATF2 (phospho T71) antibody [E268] (ab32019) at 0.526 µg/ml
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) grown in serum free media for overnight, whole cell lysate
Lane 2 : HeLa grown in serum free media for overnight, then treated with 250ng/ml Anisomycin for 30min, whole cell lysate
Lane 3 : HeLa grown in serum free media for overnight, then treated with 250ng/ml Anisomycin for 30min whole cell lysate. Then the membrane was incubated with alkaline phosphatase.
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 54 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking/Diluting Buffer and concentration: 5% NFDM /TBST
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Immunocytochemistry of HeLa (Human epithelial cell line from cervix adenocarcinoma), prepared in FBS free medium overnight labeling ATF2 at 0.9 μg/ml. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as the secondary antibody at 1/500 . Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as the counter stain at 2.5 μg/ml. DAPI was used for nuclear counter stain. Confocal image showing the expression was increased on HeLa cells, prepared in FBS free medium overnight, then treated with 250ng/ml anisomycin for 30min.
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All lanes : Anti-ATF2 (phospho T71) antibody [E268] (ab32019) at 0.1 µg/ml
Lane 1 : Untreated NIH/3T3 (mouse embryo), prepared in FBS free medium overnight, whole cell lysate
Lane 2 : NIH/3T3, prepared in FBS free medium overnight, then treated with 10 µg/ml anisomycin for 30 minutes whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking and diluting buffer used was 2% BSA/TBST.
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Lane 1 (input): HeLa (human cervix adenocarcinoma) treated with 250ng/ml anisomycin for 30min whole cell lysate 10μg.
Lane 2 (+): HeLa treated with 250ng/ml anisomycin for 30min whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32019 in HeLa treated with 250ng/ml anisomycin for 30min whole cell lysate.Ab32019 Immunoprecipitating ATF2 in Human Hela whole cell lysate. For western blotting ab32019 (1:1000) was used to confirm successful immunoprecipitation. Blocking and diluting buffer used was 5% NFDM/TBST.
All lanes : Anti-ATF2 (phospho T71) antibody [E268] (ab32019) at 0.47 µg/ml
Lane 1 : HeLa (human cervix adenocarcinoma) treated with 250ng/ml anisomycin for 30min whole cell lysate at 10 µg
Lane 2 : HeLa treated with 250ng/ml anisomycin for 30min whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab32019 in HeLa treated with 250ng/ml anisomycin for 30min whole cell lysate
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution
Exposure time: 3 minutes -
Dot blot analysis of ATF2 (phospho T71) peptide (Lane 1) and ATF2 non-phospho peptide (Lane 2) using ab32019 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.
Exposure time: 3 minutes
Blocking and Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-ATF2 (phospho T71) antibody [E268] (ab32019) at 0.1 µg/ml
Lane 1 : HeLa (human cervix adenocarcinoma), prepared in FBS free medium overnight, whole cell lysate
Lane 2 : HeLa, prepared in FBS free medium overnight, then treated with 250ng/ml anisomycin for 30 minutes whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking and diluting buffer used was 2% BSA/TBST .
The molecular weight is 70kD, consistent with the literature (PMID: 24223142).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab32019 は 9 報の論文で使用されています。
- Yuan L et al. Lateral confined growth of cells activates Lef1 dependent pathways to regulate cell-state transitions. Sci Rep 12:17318 (2022). PubMed: 36243826
- Ma Y et al. Ror2-mediated non-canonical Wnt signaling regulates Cdc42 and cell proliferation during tooth root development. Development 148:N/A (2021). PubMed: 33323370
- Lin J et al. Hepatokine Pregnancy Zone Protein Governs the Diet-Induced Thermogenesis Through Activating Brown Adipose Tissue. Adv Sci (Weinh) 8:e2101991 (2021). PubMed: 34514733
- He H et al. Copper Oxide Nanoparticles Induce Oxidative DNA Damage and Cell Death via Copper Ion-Mediated P38 MAPK Activation in Vascular Endothelial Cells. Int J Nanomedicine 15:3291-3302 (2020). PubMed: 32494130
- Hu X et al. Ubiquitin fold modifier 1 activates NF-?B pathway by down-regulating LZAP expression in the macrophage of diabetic mouse model. Biosci Rep 40:N/A (2020). PubMed: 31829413
- Huang C et al. Phosphoproteomic characterization of the signaling network resulting from activation of the chemokine receptor CCR2. J Biol Chem 295:6518-6531 (2020). PubMed: 32241914
- Yi D et al. Zc3h10 Acts as a Transcription Factor and Is Phosphorylated to Activate the Thermogenic Program. Cell Rep 29:2621-2633.e4 (2019). PubMed: 31775033
- Liu F et al. The role of NF-?B-mediated JNK pathway in cognitive impairment in a rat model of sleep apnea. J Thorac Dis 10:6921-6931 (2018). PubMed: 30746238
- Wu X et al. CUG-binding protein 1 regulates HSC activation and liver fibrogenesis. Nat Commun 7:13498 (2016). WB ; Human . PubMed: 27853137