PBS is without Mg2+ and Ca2+ and BSA is IgG and protease free.
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Immunogen affinity purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated ATF2. The final product is generated by affinity chromatography using an ATF2-derived peptide phosphorylated at threonines 69 and 71.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.35 - 1 µg/ml. Predicted molecular weight: 60 kDa.
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Abundant expression seen in the brain.
Belongs to the bZIP family. ATF subfamily. Contains 1 bZIP domain. Contains 1 C2H2-type zinc finger.
Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
Cyclic AMP-responsive element-binding protein 2 antibody
HB 16 antibody
Histone acetyltransferase ATF2 antibody
MXBP protein antibody
TREB 7 antibody
Western blot - ATF2 (phospho T71) antibody (ab4736)
Predicted band size : 60 kDa
NIH3T3 cells were left untreated (1) or treated with anisomycin (2-5), and cell lysates were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL ATF2 [pT71] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 4), the phosphopeptide immunogen (2), the non-phosphopeptide corresponding to the immunogen (3), or, a generic phosphothreonine containing peptide (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ATF2 [pT71] blocks the antibody signal, thereby demonstrating the specificity of the antibody, and anisomycin-induced threonine phosphorylation of ATF2 [pT71].