製品の概要
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製品名Anti-ATF2 (phospho S62 + S44) antibody
ATF2 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to ATF2 (phospho S62 + S44)
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由来種Rabbit
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特異性ab28847 recognises Phospho-ATF-2(Ser44)
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アプリケーション適用あり: ELISA, IP, IHC-P, WBmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic phosphopeptide derived from human ATF-2 around the phosphorylation site of Serine 62 or 44.
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ポジティブ･コントロール
- HeLa lysate; Breast carcinoma.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab28847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | 1/20000. | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
ターゲット情報
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機能Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
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組織特異性Abundant expression seen in the brain.
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配列類似性Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger. -
翻訳後修飾Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
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細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 1386 Human
- Entrez Gene: 100047997 Mouse
- Entrez Gene: 11909 Mouse
- Entrez Gene: 81647 Rat
- Omim: 123811 Human
- SwissProt: P15336 Human
- SwissProt: P16951 Mouse
- SwissProt: Q00969 Rat
see all -
別名
- Activating transcription factor 2 antibody
- Activating transcription factor 2 splice variant ATF2 var2 antibody
- ATF 2 antibody
see all
画像
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Western blot - Anti-ATF2 (phospho S62 + S44) antibody (ab28847)All lanes : Anti-ATF2 (phospho S62 + S44) antibody (ab28847)
Lane 1 : HeLa cells treated with TNF-alpha with phospho peptide
Lane 2 : HeLa cells treated with TNF-alpha
Predicted band size: 52 kDa -
Immunohistochemistry (Paraffin-embedded sections) - ATF2 antibody (ab28847)Immunohistochemistry (Paraffin-embedded sections) analysis of human breast carcinoma labeling ATF2 with ab28847.
ab28847 immunohistochemistry staining paraffin embedded breast carcinoma. -
Western blot - ATF2 (phospho S62 / 44) antibody (ab28847)All lanes : Anti-ATF2 (phospho S62 + S44) antibody (ab28847)
Lane 1 : Hela Lysate with Immunizing peptide
Lane 2 : Hela Lysate
Predicted band size: 52 kDa
参考文献
ab28847 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"