Anti-ATF2 (phospho S490 + S498) 抗体 (ab22186)

製品の概要

  • 製品名Anti-ATF2 (phospho S490 + S498) antibody
    ATF2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ATF2 (phospho S490 + S498)
  • 特異性This antibody is specific for ~74 kDa ATF2 protein phosphorylated at Ser 490/498. The antibody also recognizes the phosphorylated ~54 kDa splice form of ATF2.
  • アプリケーション適用あり: IHC-Fr, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho Ser490/498 of ATF2 (Human).

  • ポジティブ・コントロール
    • Human melanoma cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None.
    Constituents: 50% Glycerol, 0.1mg/ml BSA, 150mM Sodium chloride, 10mM HEPES. pH 7.5.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab22186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/1000.
WB 1/1000. Predicted molecular weight: 52 kDa.

ターゲット情報

  • 機能Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
  • 組織特異性Abundant expression seen in the brain.
  • 配列類似性Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
    Contains 1 C2H2-type zinc finger.
  • 翻訳後修飾Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activating transcription factor 2 antibody
    • Activating transcription factor 2 splice variant ATF2 var2 antibody
    • ATF 2 antibody
    • Atf-2 antibody
    • Atf2 antibody
    • ATF2 protein antibody
    • ATF2_HUMAN antibody
    • cAMP Response Element Binding Protein 2 antibody
    • cAMP response element binding protein CRE BP1 antibody
    • cAMP response element-binding protein CRE-BP1 antibody
    • cAMP responsive element binding protein 2, formerly antibody
    • cAMP-dependent transcription factor ATF-2 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CRE BP1 antibody
    • CRE-BP antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • CREBP1 antibody
    • Cyclic AMP dependent transcription factor ATF 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-2 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • D130078H02Rik antibody
    • D18875 antibody
    • HB 16 antibody
    • HB16 antibody
    • Histone acetyltransferase ATF2 antibody
    • MGC105211 antibody
    • MGC105222 antibody
    • MGC111558 antibody
    • MGC142504 antibody
    • mXBP antibody
    • MXBP protein antibody
    • Tg(Gzma-Klra1)7Wum antibody
    • TREB 7 antibody
    • TREB7 antibody
    see all

Anti-ATF2 (phospho S490 + S498) antibody (ab22186) 使用論文

ab22186 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HaCAT (keratinocytes))
Loading amount 50 µg
Specification HaCAT (keratinocytes)
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
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投稿 May 05 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"