製品の概要

  • 製品名
    Anti-ATF2 antibody [E242]
    ATF2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E242] to ATF2
  • 特異性
    ab32061 recognises ATF-2 (activating transcription factor-2, CRE-BP1). It does not cross-react with other ATF family members
  • アプリケーション
    適用あり: WB, ICC, IHC-P, IPmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues N-terminus of human ATF-2

  • ポジティブ・コントロール
    • HeLa cell lysate and human carcinoma
  • 特記事項

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab32061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 54 kDa).
ICC 1/250 - 1/500.
IHC-P 1/100.
IP 1/50.
  • 追加情報
    Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能
      Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
    • 組織特異性
      Abundant expression seen in the brain.
    • 配列類似性
      Belongs to the bZIP family. ATF subfamily.
      Contains 1 bZIP domain.
      Contains 1 C2H2-type zinc finger.
    • 翻訳後修飾
      Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Activating transcription factor 2 antibody
      • Activating transcription factor 2 splice variant ATF2 var2 antibody
      • ATF 2 antibody
      • Atf-2 antibody
      • Atf2 antibody
      • ATF2 protein antibody
      • ATF2_HUMAN antibody
      • cAMP Response Element Binding Protein 2 antibody
      • cAMP response element binding protein CRE BP1 antibody
      • cAMP response element-binding protein CRE-BP1 antibody
      • cAMP responsive element binding protein 2, formerly antibody
      • cAMP-dependent transcription factor ATF-2 antibody
      • cAMP-responsive element-binding protein 2 antibody
      • CRE BP1 antibody
      • CRE-BP antibody
      • CREB 2 antibody
      • CREB-2 antibody
      • CREB2 antibody
      • CREBP1 antibody
      • Cyclic AMP dependent transcription factor ATF 2 antibody
      • Cyclic AMP-dependent transcription factor ATF-2 antibody
      • Cyclic AMP-responsive element-binding protein 2 antibody
      • D130078H02Rik antibody
      • D18875 antibody
      • HB 16 antibody
      • HB16 antibody
      • Histone acetyltransferase ATF2 antibody
      • MGC105211 antibody
      • MGC105222 antibody
      • MGC111558 antibody
      • MGC142504 antibody
      • mXBP antibody
      • MXBP protein antibody
      • Tg(Gzma-Klra1)7Wum antibody
      • TREB 7 antibody
      • TREB7 antibody
      see all

    Anti-ATF2 antibody [E242] 画像

    • Anti-ATF2 antibody [E242] (ab32061) at 1/10000 dilution + HeLa cell lysate

      Predicted band size : 54 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human carcinoma using ab32061 at a dilution of 1/50

    Anti-ATF2 antibody [E242] (ab32061) 使用論文

    This product has been referenced in:
    • Li Q  et al. ATF2 translation is induced under chemotherapeutic drug-mediated cellular stress via an IRES-dependent mechanism in human hepatic cancer Bel7402 cells. Oncol Lett 12:4795-4802 (2016). Read more (PubMed: 28105187) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab32061.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録