製品の概要

  • 製品名Anti-ATF2 antibody
    ATF2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ATF2
  • 特異性ATF2 antibody detects endogenous levels of ATF2 protein around Threonine 71 or 53.
  • アプリケーション適用あり: WB, IHC-P, ELISA, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    The antiserum was produced against synthesized non-phosphopeptide derived from human ATF2 around the phosphorylation site of Threonine 71 or 53.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab47476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 52 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
  • 組織特異性Abundant expression seen in the brain.
  • 配列類似性Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
    Contains 1 C2H2-type zinc finger.
  • 翻訳後修飾Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activating transcription factor 2 antibody
    • Activating transcription factor 2 splice variant ATF2 var2 antibody
    • ATF 2 antibody
    • Atf-2 antibody
    • Atf2 antibody
    • ATF2 protein antibody
    • ATF2_HUMAN antibody
    • cAMP Response Element Binding Protein 2 antibody
    • cAMP response element binding protein CRE BP1 antibody
    • cAMP response element-binding protein CRE-BP1 antibody
    • cAMP responsive element binding protein 2, formerly antibody
    • cAMP-dependent transcription factor ATF-2 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CRE BP1 antibody
    • CRE-BP antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • CREBP1 antibody
    • Cyclic AMP dependent transcription factor ATF 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-2 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • D130078H02Rik antibody
    • D18875 antibody
    • HB 16 antibody
    • HB16 antibody
    • Histone acetyltransferase ATF2 antibody
    • MGC105211 antibody
    • MGC105222 antibody
    • MGC111558 antibody
    • MGC142504 antibody
    • mXBP antibody
    • MXBP protein antibody
    • Tg(Gzma-Klra1)7Wum antibody
    • TREB 7 antibody
    • TREB7 antibody
    see all

Anti-ATF2 antibody 画像

  • All lanes : Anti-ATF2 antibody (ab47476) at 1/500 dilution

    Lane 1 : extracts from HeLa cells.
    Lane 2 : extracts from HeLa cells preincubated with immunizing peptide


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using ATF2 antibody; Right: The same antibody preincubated with synthesized peptide.

Anti-ATF2 antibody (ab47476) 使用論文

This product has been referenced in:
  • Mamrosh JL  et al. Nuclear receptor LRH-1/NR5A2 is required and targetable for liver endoplasmic reticulum stress resolution. Elife 3:e01694 (2014). WB ; Mouse . Read more (PubMed: 24737860) »
  • White SJ  et al. Characterization of the differential response of endothelial cells exposed to normal and elevated laminar shear stress. J Cell Physiol 226:2841-8 (2011). WB . Read more (PubMed: 21302282) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry regarding ab31419 and ab47476; and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with these two antibodies.
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (endothelial)
Loading amount 5000 cells
Specification endothelial
Treatment different shear stress exposure for 24 hours
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 Mar 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"