Anti-ATF1 (phospho S63) 抗体 [EP1591(2)Y] (ab76085)

製品の概要

  • 製品名
    Anti-ATF1 (phospho S63) antibody [EP1591(2)Y]
    ATF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1591(2)Y] to ATF1 (phospho S63)
  • 特異性
    ab76085 detects ATF1 when phosphorylated at serine 63
  • アプリケーション
    適用あり: ELISA, WB, IHC-P, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding serine 63 of human ATF1

  • ポジティブ・コントロール
    • WB: HeLa cell lysate IHC-P: human breast carcinoma
  • 特記事項

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab76085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration. PubMed: 22052915
WB 1/50000 - 1/100000. Predicted molecular weight: 29 kDa.
IHC-P 1/100 - 1/250.
ICC 1/100 - 1/250.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能
      This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes.
    • 関連疾患
      Defects in ATF1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=Chromosomal aberrations involving ATF1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with FUS generates a chimeric ATF1/FUS protein. Translocation t(12;22)(q13;q12) with EWSR1 generates a chimeric ATF1/EWSR1 protein.
    • 配列類似性
      Belongs to the bZIP family. ATF subfamily.
      Contains 1 bZIP domain.
      Contains 1 KID (kinase-inducible) domain.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Activating transcription factor 1 antibody
      • ATF 1 antibody
      • atf1 antibody
      • ATF1 EWS fusion gene antibody
      • ATF1 FUS fusion gene antibody
      • ATF1_HUMAN antibody
      • cAMP dependent transcription factor 1 antibody
      • cAMP-dependent transcription factor ATF-1 antibody
      • Cyclic AMP dependent transcription factor ATF 1 antibody
      • Cyclic AMP dependent transcription factor ATF1 antibody
      • Cyclic AMP-dependent transcription factor ATF-1 antibody
      • EWS AFT1 antibody
      • FUS ATF 1 antibody
      • FUS/ATF 1 antibody
      • Protein TREB36 antibody
      • RNA binding protein activating transcription factor 1 fusion protein antibody
      • TREB 36 antibody
      • TREB36 antibody
      • TREB36 protein antibody
      see all

    画像

    • All lanes : Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085) at 1/100000 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate, treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anit rabbit HRP labelled IgG at 1/2000 dilution

      Predicted band size : 29 kDa
      Observed band size : 29 kDa
    • Immunohistochemical analysis of ATF1 in paraffin embedded human breast carcinoma tissue, using ab76085 at a 1/100 dilution.

    参考文献

    This product has been referenced in:
    • Papadopoulos D  et al. Dehydroepiandrosterone Sulfate Stimulates Expression of Blood-Testis-Barrier Proteins Claudin-3 and -5 and Tight Junction Formation via a Gna11-Coupled Receptor in Sertoli Cells. PLoS One 11:e0150143 (2016). Read more (PubMed: 26938869) »
    • Lee CJ  et al. Fibroblast and Epidermal Growth Factors Utilize Different Signaling Pathways to Induce Anchorage-independent Cell Transformation in JB6 Cl41 Mouse Skin Epidermal Cells. J Cancer Prev 19:199-208 (2014). Read more (PubMed: 25337589) »

    See all 5 Publications for this product

    レビューと Q&A

    Thank you for your email.

    The concentration of ab76085 batch:GR68333 is 0.2150 mg/ml.

    This a tissue culture supernatant and not affinity purified antibody. As the datasheet indicates ab76085 is a rabbit monoclonal antibody which is...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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