製品の概要

  • 製品名Anti-ATEB1B antibody
  • 製品の詳細
    Rabbit polyclonal to ATEB1B
  • 特異性Recognizes AtEB1b fusion protein expressed in transgenic Arabidopsis plants. The immunogen sequence is highly conserved (single amino acid substitution) in AtEB1a and AtEB1c.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Arabidopsis thaliana
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to C terminal amino acids 205-221 of Arabidopsis thaliana ATEB1B

  • ポジティブ・コントロール
    • Extract (cytosolic fraction) from the leaves of transgenic Arabidopsis seedlings expressing YFP-taged AtEB1b.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab50187 is affinity-purified using the immunizing peptide immobilized on agarose.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab50187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.
    An additional band at 50 kDa may be observed representing AtEB1b degradation products. Staining of AtEB1b in immunoblotting is specifically inhibited with the immunizing peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Microtubules (MT) play a central role in many cellular functions including: intracellular transport, maintenance of cell shape and polarity, and chromosome segregation. In most cells, the minus-ends of microtubules are anchored at the centrosome, whereas plus-ends are more dynamic and responsible for contacting targets such as cell cortex and chromosomes. Several factors regulate MT dynamics in cells. EB1 (End-Binding protein 1) proteins belong to a distinct family of accessory proteins, termed "plus-end tracking proteins" or "+TIPs", that bind to the plus-ends of microtubules and are thought to play an important role in regulating dynamics and organization of microtubules. These microtubule-associated proteins (MAPs) are among the most conserved components of the MT cytoskeleton. All EB1 family proteins have a highly conserved N-terminal region that contains a calponin homology (CH) domain and an EB1-like C-terminal motif, which is unique to EB1 family proteins.
    • 細胞内局在Cytoskeleton
    • 参照データベース
      • 別名
        • Arabidopsis thaliana EB1B antibody
        • AT5G62500 antibody
        • ATEB1A antibody
        • AtEB1B antibody
        • K19B1.11 antibody
        • K19B1_11 antibody
        • microtubule binding antibody
        see all

      Anti-ATEB1B antibody (ab50187) 使用論文

      ab50187 has not yet been referenced specifically in any publications.

      Product Wall

      There are currently no Abreviews or Questions for ab50187.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"