製品の概要

  • 製品名Anti-ATE1 antibody
    ATE1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ATE1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 432-481 (TYVWVPIEQC LPSLENSKYC RFNQDPEAVD EDRSTEPDRL QVFHKRAIMP) of Human ATE1 (NP_008972)

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab84636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway. Does not arginylate cysteine residues.
  • 配列類似性Belongs to the R-transferase family.
  • 細胞内局在Cytoplasm and Nucleus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI225793 antibody
    • Arginine tRNA protein transferase 1 antibody
    • Arginine-tRNA--protein transferase 1 antibody
    • Arginyl-tRNA--protein transferase 1 antibody
    • Arginyl-tRNA-protein transferase antibody
    • Arginyltransferase 1 antibody
    • Ate1 antibody
    • ATE1_HUMAN antibody
    • AW547406 antibody
    • EC 2.3.2.8 antibody
    • MGC26724 antibody
    • OTTHUMP00000020637 antibody
    • OTTHUMP00000058665 antibody
    • R transferase 1 antibody
    • R-transferase 1 antibody
    see all

Anti-ATE1 antibody 画像

  • Anti-ATE1 antibody (ab84636) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 59 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 63 kDa. We are unsure as to the identity of these extra bands.

Anti-ATE1 antibody (ab84636) 使用論文

ab84636 has not yet been referenced specifically in any publications.

Product Wall

I suggest trying anti-ATE1 ab90561, which is a mouse monoclonal, tested for reactivity with human Jurkat but untested on mouse samples. One issue might be reactivity of an anit-mouse IgG secondary with endogenous IgG in the mosue lysate, but this is un...

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We have two other antibodies against each of these targets which may give you a better result. However, before sending replacements, we typically collect details of the protocols from our customers before issuing a replacement or credit/refund, in t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"