製品の概要

  • 製品名Anti-ATBF1 antibody
    ATBF1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to ATBF1
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Recombinant Fragment
    交差が予測される動物種: Mouse, Human
  • 免疫原

    Recombinant fragment: EDFESPSMSS VNLNFDQTKL DNDDCSSVNT AITDTTTGDE GNADNDSATG IATETKSSS APNEGLTKAA MMAMSEYEDR LSSGLVSPAP SFYSKEYDNE G, corresponding to internal sequence amino acids 2811-2911 of Human ATBF1 with a 26 kDa tag. (NP_008816).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged ATBF1 is approximately 0.3ng/ml if used as a capture antibody.
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

ターゲット情報

  • 関連性ATBF1 is a transcription factor that negatively regulates alpha fetoprotein and MYB, transactivates CDKN1A, and may be a tumor suppressor. Loss of ATBF1 is one mechanism that defines the absence of growth control in prostate cancer. Presence of the isoform ABTF1-A is correlated with better prognosis in breast cancer and may also serve as a marker of endocrine responsiveness.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • Alpha fetoprotein enhancer binding protein antibody
    • AT binding transcription factor 1 antibody
    • AT motif binding factor antibody
    • ATBT antibody
    • ZFHX3 antibody
    see all

Anti-ATBF1 antibody 画像

  • Anti-ATBF1 antibody (ab54853) at 1/500 dilution + Recombinant tagged fragment of human ATBF1 at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

Anti-ATBF1 antibody (ab54853) 使用論文

ab54853 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Neurons)
Specification Neurons
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jun 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"