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Recombinant fragment: EDFESPSMSS VNLNFDQTKL DNDDCSSVNT AITDTTTGDE GNADNDSATG IATETKSSS APNEGLTKAA MMAMSEYEDR LSSGLVSPAP SFYSKEYDNE G, corresponding to internal sequence amino acids 2811-2911 of Human ATBF1 with a 26 kDa tag. (NP_008816).
Our Abpromise guarantee covers the use of ab54853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution. Detection limit for recombinant tagged ATBF1 is approximately 0.3ng/ml if used as a capture antibody.|
|WB||1/500 - 1/1000. Detects a band of approximately 37 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.|
ab54853 has not yet been referenced specifically in any publications.