製品の概要

  • 製品名Anti-Atad5 antibody
    Atad5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Atad5
  • アプリケーション適用あり: ICC/IF, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residues 775 and 825 of human Atad5 (NP_079133.3)

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 22493068
WB 1/2000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 208 kDa).

One of our customer have mentioned using WB protocol published in J. Biol. Chem. 2010, 285:10362-10369 with lymphoblastoid cell.

IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Involved in DNA damage response. Involved in a RAD9A-related damage checkpoint, a pathway that is important in determining whether DNA damage is compatible with cell survival or whether it requires cell elimination by apoptosis. Modulates the RAD9A interaction with BCL2 and thereby induces DNA damages-induced apoptosis.
  • 配列類似性Belongs to the AAA ATPase family.
  • 翻訳後修飾ATR may stimulate the RAD9A dissociation.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATAD5 antibody
    • ATAD5_HUMAN antibody
    • ATPase family AAA domain containing 5 antibody
    • ATPase family AAA domain-containing protein 5 antibody
    • C17orf41 antibody
    • chromosome fragility associated gene 1 antibody
    • Chromosome fragility-associated gene 1 protein antibody
    • ELG1 antibody
    • Enhanced level of genomic instability 1 homolog antibody
    • FRAG1 antibody
    see all

Anti-Atad5 antibody 画像

  • All lanes : Anti-Atad5 antibody (ab72111) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg/ml
    Lane 2 : HeLa whole cell lysate at 15 µg/ml
    Lane 3 : HeLa whole cell lysate at 5 µg/ml
    Lane 4 : 293T whole cell lysate at 50 µg/ml

    Developed using the ECL technique

    Predicted band size : 208 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of Atad5 by Western Blot of Immunprecipitate.
    ab72111 at 1µg/ml staining Atad5 in HeLa whole cell lysate immunoprecipitated using ab72111 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-Atad5 antibody (ab72111) 使用論文

This product has been referenced in:
  • Shiomi Y  et al. Two different replication factor C proteins, Ctf18 and RFC1, separately control PCNA-CRL4Cdt2-mediated Cdt1 proteolysis during S phase and following UV irradiation. Mol Cell Biol 32:2279-88 (2012). ICC/IF ; Human . Read more (PubMed: 22493068) »
  • Rahman S  et al. The Brd4 extraterminal domain confers transcription activation independent of pTEFb by recruiting multiple proteins, including NSD3. Mol Cell Biol 31:2641-52 (2011). Read more (PubMed: 21555454) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

投稿 Feb 11 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"