Anti-ATAD1 抗体 [N125/10] (ab94583)

製品の概要

  • 製品名
    Anti-ATAD1 antibody [N125/10]
    ATAD1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [N125/10] to ATAD1
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WB, IHC-P, IHC-Fr, ICC, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Fusion protein, corresponding to amino acids 1-361 (full length) of Mouse ATAD1

  • ポジティブ・コントロール
    • Rat brain lysate
  • 特記事項

    The clone number has been updated from S125-10 to N125/10, both clone numbers name the same antibody clone.

     

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    N125/10
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab94583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
IHC-Fr
ICC
ICC/IF
  • 追加情報
    ICC: Use at a concentration of 0.1 - 1 µg/ml.
    ICC/IF: Use at a concentration of 1 - 10 µg/ml.
    IHC-P: Use at a concentration of 0.1 - 1 µg/ml.
    IHC-Fr: Use at a concentration of 0.1 - 1 µg/ml.
    WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 40 kDa.
    Note: Using the lysate without boiling may provide improved results.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      ATPase that plays a critical role in regulating the surface expression of AMPA receptors (AMPAR), thereby regulating synaptic plasticity and learning and memory. Required for NMDA-stimulated AMPAR internalization and inhibition of GRIA1 and GRIA2 recycling back to the plasma membrane; these activities are ATPase-dependent.
    • 配列類似性
      Belongs to the AAA ATPase family.
    • 細胞内局在
      Peroxisome. Cell junction, synapse, postsynaptic cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 4921525H23Rik antibody
      • AAA+ ATPase Thorase antibody
      • Ab2-088 antibody
      • AFDC1 antibody
      • Atad1 antibody
      • ATAD1_HUMAN antibody
      • ATPase family AAA domain containing protein 1 antibody
      • ATPase family AAA domain-containing protein 1 antibody
      • ATPase family, AAA domain containing 1 antibody
      • ATPase Thorase antibody
      • AW107648 antibody
      • FLJ14600 antibody
      • FNP001 antibody
      • Thorase antibody
      see all

    画像

    • Western blot detection of Atad1 in Rat brain membrane tissues using ab94583 at 1:1000 dilution.

    参考文献

    ab94583 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T)
    Loading amount
    25 µg
    Specification
    293T
    Treatment
    siRNA knockdown
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    投稿 Oct 08 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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