製品の概要

  • 製品名Anti-ASS1 antibody
    ASS1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to ASS1
  • アプリケーション適用あり: IHC-P, WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Cow, Human
    交差が予測される動物種: Mouse, Rat, Dog, Orangutan
  • 免疫原

    Synthetic peptide:

    ENPKNQAPPGLYTKTQD

    (Human) from the internal region of the protein sequence according to NP_000041.2 ; NP_446464.1.

  • ポジティブ・コントロール
    • Human kidney lysates This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab77590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 47 kDa).
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • パスウェイAmino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.
    Nitrogen metabolism; urea cycle; (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline: step 1/1.
  • 関連疾患Defects in ASS1 are the cause of citrullinemia type 1 (CTLN1) [MIM:215700]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN1 usually manifests in the first few days of life. Affected infants appear normal at birth, but as ammonia builds up in the body they present symptoms such as lethargy, poor feeding, vomiting, seizures and loss of consciousness. Less commonly, a milder CTLN1 form can develop later in childhood or adulthood.
  • 配列類似性Belongs to the argininosuccinate synthase family. Type 1 subfamily.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Argininosuccinate synthase 1 antibody
    • Argininosuccinate synthase antibody
    • Argininosuccinate synthetase 1 antibody
    • ASS antibody
    • Ass-1 antibody
    • ass1 antibody
    • ASSA antibody
    • ASSY_HUMAN antibody
    • Citrulline aspartate ligase antibody
    • Citrulline--aspartate ligase antibody
    • CTLN1 antibody
    see all

Anti-ASS1 antibody 画像

  • ICC/IF image of ab77590 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween (no animal sera) for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab77590 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 donkey anti- goat (ab150133) IgG used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-ASS1 antibody (ab77590) at 0.03 µg/ml + human kidney lysate in RIPA buffer at 35 µg

    Predicted band size : 47 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • ab77590 at 2.5 µg/ml staining ASS1 in Human Kidney by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performem, antibody revealed by AP-staining.
  • IHC image of ab77590 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77590, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-ASS1 antibody (ab77590) 使用論文

This product has been referenced in:
  • Kok CY  et al. Adeno-associated Virus-mediated Rescue of Neonatal Lethality in Argininosuccinate Synthetase-deficient Mice. Mol Ther 21:1823-31 (2013). Read more (PubMed: 23817206) »

See 1 Publication for this product

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