製品の概要

  • 製品名
    Anti-ASS1 antibody [2B10]
    ASS1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2B10] to ASS1
  • アプリケーション
    適用あり: WB, ELISA, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Monkey
  • 免疫原

    Purified recombinant fragment of Human ASS1, expressed in E. Coli.

  • ポジティブ・コントロール
    • HeLa and Jurkat cells; Human ASS1 recombinant protein; A431, Raji, L1210, MOLT4, Jurkat, A549, NIH 3T3, PC12 and COS7 cell lysates.
  • 特記事項

    This product was changed from ascites to supernatant. Lot no’s high than GR119162-30 are from Tissue Culture Supernatant

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ELISA 1/10000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/200 - 1/1000.

ターゲット情報

  • パスウェイ
    Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.
    Nitrogen metabolism; urea cycle; (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline: step 1/1.
  • 関連疾患
    Defects in ASS1 are the cause of citrullinemia type 1 (CTLN1) [MIM:215700]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN1 usually manifests in the first few days of life. Affected infants appear normal at birth, but as ammonia builds up in the body they present symptoms such as lethargy, poor feeding, vomiting, seizures and loss of consciousness. Less commonly, a milder CTLN1 form can develop later in childhood or adulthood.
  • 配列類似性
    Belongs to the argininosuccinate synthase family. Type 1 subfamily.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Argininosuccinate synthase 1 antibody
    • Argininosuccinate synthase antibody
    • Argininosuccinate synthetase 1 antibody
    • ASS antibody
    • Ass-1 antibody
    • ass1 antibody
    • ASSA antibody
    • ASSY_HUMAN antibody
    • Citrulline aspartate ligase antibody
    • Citrulline--aspartate ligase antibody
    • CTLN1 antibody
    see all

Anti-ASS1 antibody [2B10] 画像



  • Predicted band size : 47 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: ASS1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: Jurkat whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab124465 observed at 47 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab124465 was shown to recognize ASS1 when ASS1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ASS1 knockout samples were subjected to SDS-PAGE. ab124465 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • ab124465, at 1/200 dilution, staining ASS1 in HeLa cells by Immunofluorescence (green). Blue: DRAQ5 fluorescent DNA dye.
  • ab124465, at 1/200 dilution, staining ASS1 in Jurkat cells (green) by flow cytometry or negative control (red).
  • ELISA using ab124465.
  • All lanes : Anti-ASS1 antibody [2B10] (ab124465) at 1/500 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : L1210 cell lysate
    Lane 4 : MOLT4 cell lysate
    Lane 5 : Jurkat cell lysate
    Lane 6 : A549 cell lysate
    Lane 7 : NIH 3T3 cell lysate
    Lane 8 : PC12 cell lysate
    Lane 9 : COS7 cell lysate


    Predicted band size : 47 kDa
  • Anti-ASS1 antibody [2B10] (ab124465) at 1/500 dilution + Human ASS1 recombinant protein (aa: 40-236)

    Predicted band size : 47 kDa

Anti-ASS1 antibody [2B10] (ab124465) 使用論文

This product has been referenced in:
  • Ohshima K  et al. Argininosuccinate Synthase 1-Deficiency Enhances the Cell Sensitivity to Arginine through Decreased DEPTOR Expression in Endometrial Cancer. Sci Rep 7:45504 (2017). Human . Read more (PubMed: 28358054) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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