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C-IHENKVKKIQKDT, corresponding to Internal sequence amino acids 182-194 of Human Asporin. (Peptide available as ab134013.)
Our Abpromise guarantee covers the use of ab31303 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).Can be blocked with Human Asporin peptide (ab134013).|
|IHC-P||Use at an assay dependent dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 20541802|
|ICC/IF||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent concentration.|
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Blocked with 5% milk for 1 hour at 27°C.
Incubated with the primary antibody diluted in blocking buffer for 12 hours at 4°C.