Anti-Asparagine synthetase 抗体 [EP282Y] (ab40850)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP282Y] to Asparagine synthetase
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Asparagine synthetase antibody [EP282Y]
Asparagine synthetase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP282Y] to Asparagine synthetase -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human Asparagine synthetase aa 500-600 (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- K562 cell lysate, human breast carcinoma tissue and human muscle tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP282Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab40850の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).
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特記事項 |
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WB
1/1000 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa). |
ターゲット情報
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パスウェイ
Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1. -
配列類似性
Contains 1 asparagine synthetase domain.
Contains 1 glutamine amidotransferase type-2 domain. - Information by UniProt
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参照データベース
- Entrez Gene: 440 Human
- Entrez Gene: 27053 Mouse
- Entrez Gene: 25612 Rat
- Omim: 108370 Human
- SwissProt: P08243 Human
- SwissProt: Q61024 Mouse
- SwissProt: P49088 Rat
- Unigene: 489207 Human
see all -
別名
- asnS antibody
- ASNS_HUMAN antibody
- ASNSD antibody
see all
画像
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Anti-Asparagine synthetase antibody [EP282Y] (ab40850) at 1/2000 dilution + K562 cell lysate at 10 µg
Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab40850 は 6 報の論文で使用されています。
- Nishikawa G et al. Targeting Asparagine Synthetase in Tumorgenicity Using Patient-Derived Tumor-Initiating Cells. Cells 11:N/A (2022). PubMed: 36291140
- Mukherjee A et al. Asparagine Synthetase Is Highly Expressed at Baseline in the Pancreas Through Heightened PERK Signaling. Cell Mol Gastroenterol Hepatol 9:1-13 (2020). PubMed: 31421261
- Restall IJ et al. Brain Tumor Stem Cell Dependence on Glutaminase Reveals a Metabolic Vulnerability through the Amino Acid Deprivation Response Pathway. Cancer Res 80:5478-5490 (2020). PubMed: 33106333
- Huang ML et al. Molecular and functional alterations in a mouse cardiac model of Friedreich ataxia: activation of the integrated stress response, eIF2a phosphorylation, and the induction of downstream targets. Am J Pathol 183:745-57 (2013). WB ; Mouse . PubMed: 23886890
- Scotti C et al. Cell-cycle inhibition by Helicobacter pylori L-asparaginase. PLoS One 5:e13892 (2010). WB, IHC-P ; Human . PubMed: 21085483
- Pos Z et al. Histamine suppresses fibulin-5 and insulin-like growth factor-II receptor expression in melanoma. Cancer Res 68:1997-2005 (2008). PubMed: 18339882